Effects of Antioxidant Nutrient Supplementation on the Lipid Peroxidation and Antioxidative Enzyme Activities in Patients with Coronary Heart Disease

  • Joung, Hyojee (Department of Culinary Science, Honam University) ;
  • Chun, Byung Yeol (Department of Preventive Medicine and Public Health, School of Medicine, Kyungpook National University) ;
  • Choi, Young Sun (Department of Food and Nutrition, Taegu University) ;
  • Kim, Sueun (Department of Food and Nutrition, Catholic University of Taegu) ;
  • Park, Wee Hyun (Department of Internal Medicine, School of Medicine, Kyungpook National University) ;
  • Jun, Jae Eun (Department of Internal Medicine, School of Medicine, Kyungpook National University) ;
  • Chae, Shung Chull (Department of Internal Medicine, School of Medicine, Kyungpook National University) ;
  • Song, Kyung Eun (Department of Clinical Pathology, School of Medicine, Kyungpook National University) ;
  • Cho, Sung Hee (Department of Food and Nutrition, Catholic University of Taegu) ;
  • Oh, Hee Sook (Department of Culinary Science, Honam University)
  • Published : 2001.03.01

Abstract

This study was carried out to evaluate whether antioxidant nutrient suppplementation with $\alpha$-tocopherol, vitamin C, $\beta$-carotene, and selenium reduces the lipid peroxide levels and increases the antioxidative enzyme activities in patients with coronary hart disease. Eighty nine patients participated in a randomized, double-blind, placebo-controlled trial. The antioxidant group (45 patients) was given daily doses of $\alpha$-tocopherol (400 IU), vitamin C (50 mg), $\beta$-carotene (15 mg), and selenium (50 $\mu\textrm{g}$) and forty four patients received a placebo. Thirty eight subjects (84.4%) of the antioxidant group and thirty nine subjects (88.6%) of the placebo group completed the three-month supplementation. Serum levels of tocopherol, vitamin C and $\beta$-carotene significantly increased in the antioxidant group compared with the baseline (p<0.05). Thiobarbituric acid-reactive substances(TBARS) decreased significantly (0.6 nmol MDA/mL) in the antioxidant group compared with that (0.09 nmol MDA/mL) in the placebo group (p=0.03). However, antioxidant supplementation did not affect the level of oxidized-LDL measured as autoantibodies against oxidized-LDL. The superoxide dimutase activity in red blood cells increased in the antioxidant group compared with the baseline (p<0.05). However, glutathione peroxidase activities did not change after supplementation in both groups, and catalase activity significantly decreased in the placebo group (p<0.05). These results suggest that antioxidant supplementation for 3 months with $\alpha$-tocopherol, vitamin C, $\beta$-carotene and selenium in patients with coronary heat disease may be partially protective against oxidative stress.