Characteristics of Antifungal Bacterium, Bacillus subtilis YS1 and It′s Mutant Induced by Gamma Radiation

온천수로부터 분리한 항진균세균의 특성 및 감마선$(Co^{60})$ 조사를 이용한 돌연변이체 유기

  • 이영근 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 김재성 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 송인근 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 정혜영 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 장화형 (배재대학교 바이오의약연구센터)
  • Published : 2001.12.01


Antifungal bacterium, Bacillus subtilis YS1 was isolated from Yusong hot spring showed broad antifungal spectrum against 12 kinds of plant pathogenic fungi and Candida albicans, animal pathogen. From the gamma($Co^{60}$) radiation sensitivity test, $D_10$ value was 2.08 kGy and it survived above 20 kGy of radiation dose. Several mutants were induced by gamma radiation. Among them, YS1-1009 mutant showed resistance against tebuconazole of herbicide, increased activity against Botryoshaeria dothidea and ligninase activity. YS67 mutant was antifungal deficient auxotrophic mutants(trp-pro-or arg-ura-). From this results, it suggested that gamma irradiation could be useful method for mutant induction.


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