Characteristics of Antifungal Bacterium, Bacillus subtilis YS1 and It′s Mutant Induced by Gamma Radiation

온천수로부터 분리한 항진균세균의 특성 및 감마선$(Co^{60})$ 조사를 이용한 돌연변이체 유기

  • 이영근 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 김재성 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 송인근 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 정혜영 (한국원자력연구소·RI·방사선응용연구팀) ;
  • 장화형 (배재대학교 바이오의약연구센터)
  • Published : 2001.12.01

Abstract

Antifungal bacterium, Bacillus subtilis YS1 was isolated from Yusong hot spring showed broad antifungal spectrum against 12 kinds of plant pathogenic fungi and Candida albicans, animal pathogen. From the gamma($Co^{60}$) radiation sensitivity test, $D_10$ value was 2.08 kGy and it survived above 20 kGy of radiation dose. Several mutants were induced by gamma radiation. Among them, YS1-1009 mutant showed resistance against tebuconazole of herbicide, increased activity against Botryoshaeria dothidea and ligninase activity. YS67 mutant was antifungal deficient auxotrophic mutants(trp-pro-or arg-ura-). From this results, it suggested that gamma irradiation could be useful method for mutant induction.

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