Identification of Total Extracellular Fibrinase from Bacillus sp. DJ Using One-or Two-Dimensional Fibrin Zymography for Proteomic Approach

  • CHOI, NACK-SHICK (Proteome Research Laboratory, Korea research Institute of Bioscience and Biotechnology) ;
  • JIN-YOUNG LEE (Proteome Research Laboratory, Korea research Institute of Bioscience and Biotechnology) ;
  • KAB-SEOG YOON (Proteome Research Laboratory, Korea research Institute of Bioscience and Biotechnology) ;
  • KYOUNG-YOEN HAN (Proteome Research Laboratory, Korea research Institute of Bioscience and Biotechnology) ;
  • SEUNG-HO KIM (Proteome Research Laboratory, Korea research Institute of Bioscience and Biotechnology)
  • Published : 2001.12.01

Abstract

An extracellular fibrinolytic-enzyme-producing bacterium was isolated from Doen-Jang, a Korean traditional fermented flood, and identified as Bacillus sp. DJ based on its morphology and cellular fatty acid composition. The total extracellular fibrinase (EF) from Bacillus sp. DJ was analyzed using three fibrin zymographic techniques, SDS-fibrin zymography (SDS-FZ), isoelectrofocucing-fibrin zymographs(IEF-FZ), and a two-dimensional SDS-fibrin zymographic analysis (2D SDS-FZ). As a result, the EP map of Bacillus sp. DJ was established. The results suggest that the 2D SDS-FZ method will be a useful tool for the proteomic approach for many other bacterial pretenses.

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