Factors Affecting the Survival of Frozen Thawed Bovine In Vitro Produced Blastocysts

  • Gustafsson, H. (Swedish Dairy Association) ;
  • Larsson, B. (Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences) ;
  • Shamsuddin, M. (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Jaakma, U. (Estonian Research Institute of Animal Breeding and Vet. Med.) ;
  • Emanuelson, U. (Department of Animal Breeding and genetics, Swedish University of Agricultural Sciences)
  • Received : 2000.01.05
  • Accepted : 2000.07.10
  • Published : 2001.01.01


Factors Affecting the Survival of Frozen Thawed Bovine In Vitro Produced Blastocysts. The effect of some factors on the post-thaw survival of a total of 240 in vitro produced bovine blastocysts was investigated using logistic regression analysis. The explanatory variables tested were: type of culture medium before freezing (TCM 199 supplemented with BSA, BSAITS (BSA+insulin+transferrin+selenium), ECS (estrous cow serum) with or without BOEC (bovine oviductal epithelial cells), age of the blastocyst (Day 7, Day 8+9), morphological appearance before freezing (distinct=Q1 or indistinct=Q2 inner cell mass) and type of cryoprotectant (glycerol, 1.0 M or ethylene glycol, 1.6 M). The survival after thawing based on the post-thaw quality and the development after co-culture with BOEC for 24 and 48 hours. Day 7 blastocysts had an almost three times better chance of survival than Day 8+9 blastocysts. Q1, Day 8+9 blastocysts had higher odds to survive after 48 hours in culture than Q2 blastocysts (p<0.05). Blastocysts produced in BSAITS medium had the best chances of survival; however, the odds were not always significant. Blastocysts frozen in glycerol had a better post-thaw quality rating than those frozen in ethylene glycol; however, the difference in post-thaw development at culture was not significant. The relationship between post-thaw quality and post-thaw development at culture was significant (p<0.05). The developmental stage and/or age of the embryo and culture medium where development up to blastocyst takes place affect the post-thaw survival of the bovine embryos.


Bovine;Embryo Age;Insulin-Transferrin-Selenium;Glycerol;Ethylene Glycol;Post-Thaw Quality Score

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