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Cloning and Expression of β1-Adrenergic Receptor Genes in Adipose Tissues from Korean Native Cattle (Hanwoo)

  • Ha, S.H. (School of Agricultural Biotechnology, Seoul National University) ;
  • Chung, M.I. (School of Agricultural Biotechnology, Seoul National University) ;
  • Baik, M.G. (Department of Genetic Engineering and Biotechnology Research Institute, Chonnam National University) ;
  • Choi, Y.J. (School of Agricultural Biotechnology, Seoul National University)
  • Received : 2000.03.10
  • Accepted : 2000.08.30
  • Published : 2001.01.01

Abstract

Bovine ${\beta}1$-adrenergic receptor (AR) cDNA was cloned using degenerative primers. Bovine ${\beta}1$-AR coded for 467 amino acids and the comparison of the deduced amino acid sequence with that of sheep showed 93.4% identity. Northern blot analysis indicated that transcript size for the bovine ${\beta}1$-AR was 3.6 kb in the adipose tissue. The expression level of three $\beta$-ARs (1, 2, and 3) in bovine abdominal, subcutaneous, and perirenal adipose tissues were examined using reverse transcription-polymerase chain reaction (RT-PCR), and the levels of ${\beta}1$- and ${\beta}3$-AR mRNA were found to be lower in the subcutaneous adipose tissue than in the abdominal and perirenal adipose tissues. These results suggest that the expression of $\beta$-ARs mRNA are differentially regulated among the adipose tissues.

Keywords

${\beta}1$-Adrenergic Receptor cDNA;Cloning;Expression;Adipose Tissue