Immunohistochemical localization of protein kinase C and nitric oxide synthase in the vomeronasal organ of the horse

말 서골코기관에서 protein kinase C 및 nitric oxide synthase의 면역조직학적 관찰

  • Lee, Kwanghyup (Department of Veterinary Medicine, Cheju National University) ;
  • Ahn, Meejung (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Yongduk (Department of Veterinary Medicine, Cheju National University) ;
  • Ha, Theyoung (Department of Veterinary Medicine, Cheju National University) ;
  • Kim, Heeseok (Department of Veterinary Medicine, Cheju National University) ;
  • Shin, Thekyun (Department of Veterinary Medicine, Cheju National University)
  • 이광협 (제주대학교 농과대학 수의학과) ;
  • 안미정 (제주대학교 농과대학 수의학과) ;
  • 이용덕 (제주대학교 농과대학 수의학과) ;
  • 하태영 (제주대학교 농과대학 수의학과) ;
  • 김희석 (제주대학교 농과대학 수의학과) ;
  • 신태균 (제주대학교 농과대학 수의학과)
  • Accepted : 2001.08.17
  • Published : 2001.09.25

Abstract

The expression of protein kinase C(PKC) isoforms and nitric oxide synthase (NOs) isoforms was studied in the equine vomeronasal organ(VNO), a pheromone receptor organ, using immunohistochemistry. All PKC isoforms including PKC $\alpha$, ${\beta}I$, $\delta$, and $\theta$ were detected in the supporting cells, sensory receptor cells, and basal sensory epithelial cells, while constitutive PKC $\alpha$ and ${\beta}I$ were stained more intensely than novel PKC $\delta$ and ${\theta}$. There was also a varying degree of immunostaining for PKCs in the glandular acini and VNO nerve. Constitutive neuronal and endothelial NOSs, and inducible NOS were detected in the VNO sensory epithelia. There was intense immunoreactivity for endothelial NOS in the VNO sensory epithelia but weak reactivity for neuronal NOS, while inducible NOS showed little immunoreactivity in the adjacent section. These findings suggest that both PKCs and NOSs may be involved in the process of pheromone reception in the horse. Constitutive isoforms of these enzymes may play a more important role in signal trasduction in the VNO of the horse.

Acknowledgement

Supported by : 제주대학교(동물과학연구소)