Occurrence of postweaning multisystemic wasting syndrome in pigs in Jeju

제주지역 양돈장 자돈에서 발생한 이유후 전신성 소모성 증후군의 증례

  • Kang, Jong-chul (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University) ;
  • Jeong, Kyong-ju (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University) ;
  • Ahn, Mee-jung (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University) ;
  • Lee, Du-sik (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University) ;
  • Kang, Wan-chul (Animal Health Department of Jeju Provincial Institute for Livestock Promotion) ;
  • Kim, Jin-hoe (Animal Health Department of Jeju Provincial Institute for Livestock Promotion) ;
  • Shin, Tae-kyun (Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University)
  • 강종철 (제주대학교 수의학과) ;
  • 정경주 (제주대학교 수의학과) ;
  • 안미정 (제주대학교 수의학과) ;
  • 이두식 (제주대학교 수의학과) ;
  • 강완철 (제주도 축산진흥원 가축위생시험과) ;
  • 김진회 (제주도 축산진흥원 가축위생시험과) ;
  • 신태균 (제주대학교 수의학과)
  • Accepted : 2001.06.14
  • Published : 2001.09.25

Abstract

Multiplex PCR and immunohistochemistry were used to detect and differentiate between porcine circovirus (PCV) type-I and the PCV associated with postweaning multisystemic wasting syndrome (PMWS). Unique DNA product to PCV type-II was confirmed the some organs including lymph nodes, tonsil and spleen from eight pigs in Jeju by multiplex PCR. In this study, the samples were tested by a multiplex PCR assay to determine the type of PCV in each case; all cases were PCV type-II positive. PCV type-II was identified not only in typical PMWS cases, but also in field cases submitted with various clinical histories, some of which were not suggestive of PMWS. Immunohistochemically PCV type-II antigen was detected in macrophage-like cells in the tonsil, liver, lymph nodes and spleen, while some hepatocytes and renal tubular epithelial cells were also positive to the virus. This study suggested that PCV type-II is one of the causative agents of PMWS as well as the major type of PCV in the affected pigs in Jeju.