Establishment of diagnositc method for bovine neosporosis by PCR using paraffin block

파라핀 블록 PCR을 이용한 소 네오스포라 감염증의 진단법 확립

  • Lee, Jung-keun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jae-Hoon (National Veterinary Research Institute and Quarantine Service) ;
  • Kim, Jin-Hyun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Byung-chun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Hwang, Woo-suk (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Youn, Hee-jeong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Nam, Hoo-woo (Department of Parasitology, College of Medicine, Catholic University) ;
  • Jean, Young-wha (National Veterinary Research Institute and Quarantine Service) ;
  • Kim, Dae-Yong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
  • 이중근 (서울대학교 수의과대학, 농생명공학부) ;
  • 김재훈 (국립수의과학검역원) ;
  • 김진현 (서울대학교 수의과대학, 농생명공학부) ;
  • 이병천 (서울대학교 수의과대학, 농생명공학부) ;
  • 황우석 (서울대학교 수의과대학, 농생명공학부) ;
  • 윤희정 (서울대학교 수의과대학, 농생명공학부) ;
  • 남호우 (카톨릭의과대학 기생충학교실) ;
  • 진영화 (국립수의과학검역원) ;
  • 김대용 (서울대학교 수의과대학, 농생명공학부)
  • Accepted : 2001.08.23
  • Published : 2001.09.25

Abstract

Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. Here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inflammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less sensitive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection.

Acknowledgement

Supported by : 농림부