Development of ELISA for brucella abortus RB51 I. Analysis on antigens of Brucella abortus RB51 by Westeren blot

부루세라 RB51의 ELISA 진단법개발 I. Westeren blot에 의한 Brucella abortus RB51균의 항원 분석

  • Her, Moon (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Cho, Dong-hee (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Jung, Byeong-yeal (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Cho, Seong-kun (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Jung, Suk-chan (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Kim, Ok-kyung (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services)
  • 허문 (국립수의과학검역원 세균과) ;
  • 조동희 (국립수의과학검역원 세균과) ;
  • 정병열 (국립수의과학검역원 세균과) ;
  • 조성근 (국립수의과학검역원 세균과) ;
  • 정석찬 (국립수의과학검역원 세균과) ;
  • 김옥경 (국립수의과학검역원 세균과)
  • Accepted : 2000.12.04
  • Published : 2001.03.20

Abstract

As compared with reaction of antibody for sonicated antigen of Brucella abortus strain RB51 and 1119-3 by Western blot analysis, Brucella field positive sera was detected strong reaction at 40~80 kDa LPS of strain 1119-3, but detected very weak reaction at strain RB51 partly. Otherwise, as we analyzed major immunogen of RB51 by antisera bled periodically during 6 months after RB51 vaccination. we detected strong immunological reaction at 17, 18 and 8 kDa antigen of RB51. Especially, reaction of 8 kDa antigen by Western blot coincided with reaction of dot-blot assay in RB51-antibody detection method. We also compared with reaction of field sera by STAT(standard tube agglutination test), dot-blot assay and Western blot (reaction of 8 kDa antigen of strain RB51). 16 sera of 4~5 months after RB51 vaccination are all negative by STAT, and 12 field brucellosis positive serum are all positive, and also 12 of 16 sera vaccinated RB51 are positive by dot-blot assay and reaction of 8kDa antigen by Western blot. but 1 of 15 Brucellosis negative sera reacted nonspecifically dot-blot assay.