An immunohistochemical study of endocrine cells in the gut of the Prussian carp, Carassius auratus

붕어(Carassius auratus) 장 내분비세포에 관한 면역조직화학적 연구

  • Lee, Hyeung-Sik (Department of Biology, Faculty of Natural Sciences, Kyungsan University) ;
  • Ku, Sae-Kwang (Pharmacology & Toxicology Lab., Central Research Laboratories, Dong-Wha Pharm Ind. Co.) ;
  • Park, Gi-Dae (Department of Histology, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Jae-Hyun (Department of Histology, College of Veterinary Medicine, Kyungpook National University)
  • 이형식 (경산대학교 자연과학부 생물학과) ;
  • 구세광 (동화약품(주) 중앙연구소 약리독성연구실) ;
  • 박기대 (경북대학교 수의과대학 조직학교실) ;
  • 이재현 (경북대학교 수의과대학 조직학교실)
  • Accepted : 2001.12.03
  • Published : 2001.12.24

Abstract

The regional distribution and relative frequency of neurohormonal peptides-producing cells were demonstrated in the gut of the stomachless teleost, the Prussian carp, Carassius auratus Linnaeus, using 10 types of specific antisera raised against mammalian regulatory peptides. The gut of the Prussian carp was divided into five portions from proximal to distal (Segments I~V). Most of immunoreactive cells in the epithelial lining portion, between epithelial cells, were generally spherical or spindle shape having long cytoplasmic process that reached to the lumen (open typed cell) while cells showing round in shape (close typed cell) were found in the basal portions of epithelial lining occasionally. Somatostatin-, cholecystokinin (CCK)-8- and pancreatic polypeptide (PP)- immunoreactive cells were observed in this study. However, no serotonin-, glucagon-, chromogranin A-, secretin-, vasoactive intestinal peptide (VIP)-, substance P- and bombesin-immunoreactive cells were found. Somatostatin-immunoreactive cells were restricted to most proximal segments of the gut (Segment I) with rare frequency and CCK-8-immunoreactive cells were demonstrated in the proximal segments of the gut (Segments I and II) with a few to rare frequencies. In addition, pancreatic polypeptide-immunoreactive cells were demonstrated in the proximal to middle segments (Segments I~III) with moderated to rare frequencies. In conclusion, the distribution and relative frequency of these immunoreactive cells are well corresponded to the previous reports in stomachless teleost but somewhat peculiar patterns are also detected.