Involvement of Ca2+ and K+ channels in the action of NO on gastric circular muscle

기니피그 유문부 윤상근의 자발적 수축 및 서파에 대한 nitric oxide의 억제적 작용과 Ca2+ 및 K+ 통로의 관련성

  • Kim, Tae-wan (Department of Physiology, College of Veterinary Medicine, Seoul National University) ;
  • La, Jun-ho (Department of Physiology, College of Veterinary Medicine, Seoul National University) ;
  • Yang, Il-suk (Department of Physiology, College of Veterinary Medicine, Seoul National University)
  • 김태완 (서울대학교 수의과대학 생리학교실) ;
  • 라준호 (서울대학교 수의과대학 생리학교실) ;
  • 양일석 (서울대학교 수의과대학 생리학교실)
  • Accepted : 2001.09.30
  • Published : 2001.12.24

Abstract

It was investigated whether $Ca^{2+}$ and $K^+$ channels were involved in the inhibitory action of nitric oxide (NO) on the contractile and slow wave activity of guinea pig gastric antral circular muscle. The gastric antral circular muscle showed spontaneous phasic contraction and slow wave. NO donors, 3-morpholinosydnonimine hydrochloride (SIN-1, $0.01{\sim}100{\mu}M$) and S-nitroso-L-cysteine (CysNO, $0.001{\sim}10{\mu}M$), reduced not only the amplitude of phasic contraction but also that of slow wave in a concentration-dependent manner. Both the perfusion of $Ca^{2+}$-free solution and the administration of $Ni^{2+}$, a nonselective $Ca^{2+}$ channel blocker, reduced the phasic contraction as well as the amplitude and frequency of the slow wave. The effects of these treatments were similar to those of NO donors. Nifedipine ($10{\mu}M$), a specific L-type $Ca^{2+}$ channel blocker, abolished the phasic contraction and remarkably reduced the plateau of slow wave but had no profound effect on the upstroke of slow wave. In the whole-cell patch clamp mode, CysNO shifted the steady-state activation curve for L-type $Ca^{2+}$ current to the right and the steady-state inactivation curve to the left. Pretreatment of various $K^+$ channel blockers such as tetraethylammonium (1 mM), 4-aminopyridine (0.5 mM), glibenclamide (10 mM), apamin ($0.1{\mu}M$), and iberiotoxin ($0.1{\mu}M$) did not affect the inhibitory action of SIN-1. These results suggest that NO donors suppress mechanical and electrical activity of guinea pig gastric antral circular muscle by inhibition of L-type $Ca^{2+}$ channel rather than by activation of $K^+$ channels.

Acknowledgement

Supported by : 서울대학교, 수의과대학 부속 수의과학연구소