Identification and Antimicrobial Susceptibility of Microorganisms Isolated from Bovine Mastitic Milk

젖소의 유방염 원인균 분리 및 약제 감수성 검사

  • Kang, Hee-Jung (Jeju Livestock Promotion Institute) ;
  • Kim, Ik-Chun (Jeju Livestock Promotion Institute) ;
  • Kim, Jin-Hoe (Jeju Livestock Promotion Institute) ;
  • Son, Won-Geun (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Du-Sik (Department of Veterinary Medicine, Cheju National University)
  • Accepted : 2001.11.20
  • Published : 2001.12.24

Abstract

Microorganisms were isolated and identified from bovine 296 quarters which showed positive reaction by California Mastitis Test (CMT) in 40 farms of Jeju from September 1999 to June 2000. The organisms associated with the mastitis of bovine were 11 different bacterial species in this study. Which of them, Staphylococcus aureus was the most predominant species as 152 (51.4%) isolates. Other identified species included 49 (16.5%) coliform, 47 (15.8%) Streptococcus dysgalactiae, 15 (5.1%) Bacillus spp., 8 (2.7%) Staphylococcus epidermidis, 6 (2.1%) Streptococcus agalactiae, 5 (1.7%) Enterococcus faecalis, 5 (1.7%) Corynebacterium spp., 3 (1.0%) Streptococcus uberis, 1 (0.3%) Pseudomonas aeruginosa and 1 (0.3%) Pasteurella haemolytica. Almost of all the islolated beacterial species showed high sensitivity against kanamycin (98.6%), cephalothin (98.0%), streptomycin (94.9%), gentamicin (94.6%), ampicillin (92.2%) and polymyxin B (90.2%). On the contrary, they showed resistance against penicillin (47.0%), tetracycline (37.2%), cefazolin (26.0%), bacitracin (22.6%) and erythromycin (19.9%). Eighty-one isolates were not resistant to any antibiotics and 215 drug resistant isolates showed 89 different drug resistance patterns from single to nine multiple antibiotics resistance patterns.