Study on mechanism of multistep hepatotumorigenesis in rat : Bio-indices on hepatic tumorigenesis

간암의 다단계 발생기전에 관한 연구: 종양형성 과정에서의 생체지표

  • Kang, Chung-boo (Department of Internal Medicine, College of Veterinary Medicine(Institute of Animal Medicine)) ;
  • Kim, Chi-kyeong (Department of Internal Medicine, College of Veterinary Medicine(Institute of Animal Medicine)) ;
  • Song, Seung-hee (Department of Internal Medicine, College of Veterinary Medicine(Institute of Animal Medicine)) ;
  • Ha, Woo-song (Department of Surgery, College of Medicine, Gyeongsang National University)
  • 강정부 (경상대학교 수의과 대학(동물의학 연구소)) ;
  • 김지경 (경상대학교 수의과 대학(동물의학 연구소)) ;
  • 송승희 (경상대학교 수의과 대학(동물의학 연구소)) ;
  • 하우송 (경상대학교 의과대학)
  • Accepted : 2001.10.10
  • Published : 2001.12.24

Abstract

To estalish bio-indices for detection of the development of multistep hepatotumorigenesis, rats were fed water containing 0.01% diethylnitrosamine (DEN) ad libitum for 13-14 weeks. Hepatocellular carcinoma was developed by treatment with DEN, DEN only was able to induce hepatic tumors in rats without any other cocarcinogen. Compared to control group, liver cytosol protein concentration in all treated grous was significantly decreased (p<0.05). From week to week, $20{\alpha}$-hydroxysteroid dehydrogenase ($20{\alpha}$-HSD) activity was increased and the highest activity was observed on the 12th week (p<0.05). In addition, the urine biopterin concentration was also significantly increased compared to control groups (p<0.05) in a time course manner. These results indicated that $20{\alpha}$-HSD activity, urine biopterin and liver cytosol protein concentration might be very useful maker to hepatic tumorigenesis.

Acknowledgement

Supported by : 한국 학술 진흥재단