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The Effects of Bee Venom on PLA2, COX-2, iNOS, AA and PG in RAW 264.7 Cells

봉약침액(蜂藥鍼液)이 PLA2, COX-2, iNOS, AA 및 미치는 영향(影響)에 관(關)한 연구(硏究)

  • Ha, Seang-Jong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University) ;
  • Lee, Seong-No (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University) ;
  • Jo, Hyun-Chul (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-Won University) ;
  • Kim, Kee-Hyun (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University)
  • 하성종 (경원대학교 한의과대학 침구학교실) ;
  • 이성노 (경원대학교 한의과대학 침구학교실) ;
  • 조현철 (경원대학교 한의과 대학 한방재활의학과 교실) ;
  • 김기현 (경원대학교 한의과대학 침구학교실)
  • Published : 2002.12.01

Abstract

Objectives : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide-induced expression phospholipase $A_2$, cyclooxygenase-2 and inducible nitrogen oxide synthase, and the generation of arachidonic acid, prostaglandin D2 and E2 in RAW 264.7 cells, a murine macrophage cell line. Methods : The expression of phospholipase $A_2$, cyclooxygenase and inducible nitrogen oxide synthase was determined by western blotting with corresponding antibodies, and the generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ was assayed by ELISA method in RAW 264.7 cells. The non-toxic concentrations (0.1 to $5\;{\mu}g/ml$) of bee venom determined by MTT assay, were used in this study. Results : 1. Bee venom inhibited lipopolysaccharide-induced expression of phospholipase $A_2$ in a dose dependent manner after 48 hours treatment. 2. Bee venom inhibited lipopolysaccharide-induced expression of cyclooxygenase-2 in a dose dependent manner after 24 and 48 hours treatment. 3. Bee venom inhibited lipopolysaccharide-induced expression of inducible nitrogen oxidesynthase in a dose dependent manner after 48 hours treatment. 4. The generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ was not much affected by the treatment of bee venom on the lipopolysaccharide-induced generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ in RAW 264.7 cells.

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