Cytotoxic Effects of Methanol Extract and Fractions from Echinacea angustifolia on Cancer Cells

암세포에 대한 Echinacea angustifolia 순차 용매 추출물의 세포독성 효과

  • Lee, Joon-Kyoung (Department of Food & Nutrition, Kyunghee University) ;
  • Koo, Sung-Ja (Department of Food & Nutrition, Kyunghee University)
  • 이준경 (경희대학교 식품영양학과) ;
  • 구성자 (경희대학교 식품영양학과)
  • Published : 2002.02.01


Echinacea is a North American native medicinal herb used traditionally for wounds, burns, snake or insect bites, colds, infections, and inflammation by indigenous Americans. We investigated the effects of the root and stem of fresh Korean-grown Echinacea angustifolia methanol extracts and fractionation extracts on the cytotoxicity against cancer cells (HL60, 3LL). The extracts were prepared by step-wise fractionation of methanol extracts of Echinacea angustifolia using hexane, chloroform, ethyl acetate, buthanol, and water. From the tests, root and stem parts of Echinacea showed the cytotoxic effect on cancer cells. The cytotoxie rate of the ethyl acetate fraction of the root parts showed 79% against HL60 cells at low concentration (0.125 mg/mL), and hexane fraction of the root and stem parts gradually increased as the concentration of samples increased, and the root parts showed 82% at 1.0 mg/mL concentration against HL60 cells, chloroform fraction of the root part showed 78.4% against HL60 cells and 68.4% on 3LL cells at 1.0 mg/mL concentration, water and butanol fraction of these results, it is considered that ethyl acetate fraction of the root and stem parts showed 60.1% to 77.1% against HL60 cells, after testing by MTT assay system. From these results, it is considered that ethyl acetate fraction of the Echinacea angustifolia root parts has stronger anticancer effects than any other fractions in vitro.


Echinacea angustifolia;cytotoxic effects;HL60 cells;3LL cells


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