Characterization of Bacillus polyfermenticus SCD for Oral Bacteriotherapy of Gastrointestinal Disorders

식품용 프로바이오틱 비스루트균의 주요 생균제 특성

  • Published : 2002.02.01


Bacillus polyfermenticus SCD, which is commonly called as Bisroot strain, is being used for functional foods through the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine in both humans and animals. The cells of B. polyfermenticus SCD were treated for 24 h in artifical bile after incubation for 2 h in artificial gastric juice and final number of the strain was reached to around $3.3{times}10^7\;CFU/mL$. In test of API ZYM kit, ${\beta}-glucuronidase$ or ${\beta}-glucosidase$ was not produced by B. polyfermenticus SCD. B. polyfermenticus SCD was resistant to antibiotics, such as nisin, streptomycin, tetracycline, and rifamycin. B. polyfermenticus SCD was also affected by alcohol concentration up to 4%, but more than 8%, their growth was not affected significantly. Finally, B. polyfermenticus SCD was shown to inhibit the growth of Listeria monocytogenes ATCC 19111 completely within 24 h of incubation, which indicated its bactericidal nature.


  1. Tannock, G.W. Probiotic properties of lactic acid bacteria: plenty of scope for fundamental R&D. Trends. Biotechnol. 15: 270-274 (1997)
  2. Fuller, R. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365-378 (1989)
  3. Goldin, B.R. and Borbach, S.L. Alterations in fecal microflora enzymes related to diet, age, lactobacillus supplements, and dime-thylhydrazine. Cancer40: 2421-2426 (1977)<2421::AID-CNCR2820400905>3.0.CO;2-I
  4. Gilliland S.E. and Walker, D.K. Factors to consider when select-ing a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73: 905-911 (1990)
  5. Fuller, R. Probiodcs in human medicine. Gut 32: 439-442 (1991)
  6. Hilton, E., Henry, D.I., Phyllis, A., Kenneth, F. and Michael, T.B. Ingestion of yoghurt containing Lactobacillus acidophilus as pro-phylaxis for candidal vaginitis. Ann. Int. Med. 116: 353-357 (1992)
  7. Shah, N. Lactobacillus acidophitus and lactose intolerance, a review. ASEAN Food J. 9: 47-54 (1994)
  8. Matsuzaki, T., Hashimoto, S. and Yokokura, T. Effects on antitu-mor activity and cytokine production in the thoracic cavity by intrapleural administration of Lactobacillus casei in tumor-beahng mice. Med. Microbiol. Immunol. Berl. 185: 157-161 (1996)
  9. Takiguchi, R., Mochizuki, E., Suzuki, Y., Nakajima, I. and Benno, Y. Lactobacillus acidophilus SBT 2062 and Bifidobacterium lon-gum SBT 2928 on harmful intestinal bacteria. J. Int. Microbiol. 11: 11-17 (1997)
  10. Park, H.S., Lee, S.H. and Uhm, T.B. Selecdon of microorganisms for probiotics and their charactenzation. J. Food Sci. Nutr. 3: 433-440 (1998)
  11. Green, D.H., Wakeley, P.R., Page, A., Bames, A., Baccigalupi, L., Ricca, E. and Cutting, S.M. Characterization of two Bacillus pro-biotics. Appl. Environ. Microbiol. 65: 4288-4291 (1999)
  12. Hoa, N.T., Baccigalupi, L., Huxham, A., Smertenko, A., Van, P.H., Ammendola, S., Ricca, E. and Cutting, S.M. Characteriza-tion of Bacillus species used for oral bactenotherapy and bacteri-oprophylaxis of gastrointestmal disorders, Appl. Environ, Microbiol. 66: 5241-5247 (2000)
  13. Jun, K.-D, Lee, K.-H., Kim, W.-S. and Paik, H.-D. Microbiologi-cal identiScation of medical probiotic bispan strain. Kor. J. Appl. Microbiol. Biotechnol. 28: 124-127 (2000)
  14. Paik, H.-D., Jun, K.-D., Sung, S.-H., Kim, W.-S., Kim, H.-S. and Lee, B.-C. Liquid cultivation of strains of Bacillus polyfermenti-cus. United States Patent 6, 010, 898 (1999)
  15. Kobayashi, Y., Tohyama, K. and Terashima, T. 1974. Tolerance of the multiple antibiotic resistant strain, L. casei PSR 3002, to artificial digestive fluids. Jpn. J. Microbiol. 29: 691-697 (1974)
  16. Lee, S.H. and No, M.J. Viability in artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from Kimchi. J. Appl. Microbiol. Biotechnol. 6: 617-622 (1997)
  17. Conway, P.L., Gorbach, S.L. and Goldin, B.R. Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J. Dairy Sci. 70: 1-12 (1987)
  18. Giannella, R.A., Broitman, S.A. and Zamcheck, N. Gastric acid barrier to ingested microorganisms in man: Studies in vivo and in vitro. Gut 13: 251-256 (1972)
  19. Berrada, N., Lemeland, J.R, Laroche, G., Thouvenot, P. and Piaia, M. Bifidobacterium from fermented milks: Survival during gastric transit. J. Dairy Sci. 74: 409-413 (1991)
  20. Gilliland, S.E., Staley, T.E. and Bush, L.J. Importance of bile tol-erance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67: 3045-3051 (1984)
  21. Cole, C.B., Fuller, R. and Carter, S.M. Effect of probiotic supple-ments of Lactobacillus acidophitus and Bifidobacterium adotes-centis 2204 on $\beta$-glucosidase and $\beta$-glucuronidase acdvity in the lower gut of rats associated with a human fecal flora. Microb. Ecol. Health Dis. 2: 223-225 (1989)
  22. Shin, M.-S., Kim, H.-M., Kim, G.-T., Huh, C.-S., Bae, H.-S. and Baek, Y.-J. Selection and charactenstics of Lactobacillus acido-philus isolated from Korean feces. Korean J. Food Sci. Technol. 31: 495-501 (1999)
  23. Kim, H.-J. Inhibition of spoilage and pathogenic bacteria by novel bactenocins. MS thesis, Kyungnam university (1998)
  24. Lee, K.-H., Jun, K.-D., Kim, W.-S. and Paik, H.-D. Partial char-acterization of polyfermenticin SCD, a newly identified bacteno-cin of Bacillus polyfermenticus. Lett. Appl. Microbiol. 32: 1-6 (2001)