Detection Characteristics of TL, ESR and DNA Comet for Irradiated Soybeans

열발광, 전자스핀공명 및 DNA Comet 분석에 의한 대두의 방사선 조사 여부 검지 특성

  • Lee, Eun-Young (Department of Food Science and Technology, Kyungpook National University) ;
  • Jeong, Jae-Young (Department of Food Science and Technology, Kyungpook National University) ;
  • Noh, Jung-Eun (Department of Food Science and Technology, Kyungpook National University) ;
  • Jo, Deok-Jo (Department of Food Science and Technology, Kyungpook National University) ;
  • Kwon, Joong-Ho (Department of Food Science and Technology, Kyungpook National University)
  • Published : 2002.02.01


The detection characteristics of gamma-irradiated $(0{\sim}4\;kGy)$ soybeans produced in Korea and China were investigated by thermoluminescene (TL), electron spin resonance (ESR), and DNA comet assay. The TL glow curves were shown at around $200^{\circ}C$ for irradiated soybeans, while that at $280^{\circ}C$ for the non-irradiated one. The normalization with a re-irradiation step at 1 kGy could verify the above detection results. The Korean soybean showed higher glow curves than Chinese did. The ESR spectroscopy for husks of irradiated soybeans revealed specific signals (g = 2.02374, 1.98715) derived from cellulose radical, which intensities were proportional to irradiation does, with the higher peaks in Chinese sample than Korean one. The DNA comet for the non-irradiated sample showed no or little tails, while those for irradiated samples above 0.5 kGy were remarkably changed in their length, size, and concentration, thus resulting in distinguishing non-irradiated from irradiated samples. As a result, TL, ESR, and DNA comet determinations were found suitable for the detection of irradiated soybean at 0.5 kGy or more, and negligible differences were observed between Korean and Chinese origins in their detection characteristics.


soybean;origin;thermoluminescence;electron spin resonance;DNA comet


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