Detection Characteristics of TL, ESR and DNA Comet for Irradiated Soybeans

열발광, 전자스핀공명 및 DNA Comet 분석에 의한 대두의 방사선 조사 여부 검지 특성

  • Lee, Eun-Young (Department of Food Science and Technology, Kyungpook National University) ;
  • Jeong, Jae-Young (Department of Food Science and Technology, Kyungpook National University) ;
  • Noh, Jung-Eun (Department of Food Science and Technology, Kyungpook National University) ;
  • Jo, Deok-Jo (Department of Food Science and Technology, Kyungpook National University) ;
  • Kwon, Joong-Ho (Department of Food Science and Technology, Kyungpook National University)
  • Published : 2002.02.01

Abstract

The detection characteristics of gamma-irradiated $(0{\sim}4\;kGy)$ soybeans produced in Korea and China were investigated by thermoluminescene (TL), electron spin resonance (ESR), and DNA comet assay. The TL glow curves were shown at around $200^{\circ}C$ for irradiated soybeans, while that at $280^{\circ}C$ for the non-irradiated one. The normalization with a re-irradiation step at 1 kGy could verify the above detection results. The Korean soybean showed higher glow curves than Chinese did. The ESR spectroscopy for husks of irradiated soybeans revealed specific signals (g = 2.02374, 1.98715) derived from cellulose radical, which intensities were proportional to irradiation does, with the higher peaks in Chinese sample than Korean one. The DNA comet for the non-irradiated sample showed no or little tails, while those for irradiated samples above 0.5 kGy were remarkably changed in their length, size, and concentration, thus resulting in distinguishing non-irradiated from irradiated samples. As a result, TL, ESR, and DNA comet determinations were found suitable for the detection of irradiated soybean at 0.5 kGy or more, and negligible differences were observed between Korean and Chinese origins in their detection characteristics.

Keywords

soybean;origin;thermoluminescence;electron spin resonance;DNA comet

References

  1. WHO Wholesomeness of Irradiated Pood (Report of a joint FAO/IAEA/ WHO expert committee), Technical Report Seiies-659, 7-34 (1981)
  2. IAEA homepage. www.iaea.org/icgfi (2001)
  3. Kwon, J.H., Chung, H.W. and Kwon, Y.J. Infrastmcture of quar-antine procedures for promoting the trade of irradiated foods. Paper presented at Symposium of The Korean Society of Posthar-vest Science and Technology of Agricultural Products on Irradia-tion Technology for the Safety of Food and Public HealthIndustries and Quality Assurance. Daejon, 13 October, pp. 209-254 (2000)
  4. Lee, E.Y, Kim, M.O., Lee, H.J., Kim, K.S. and Kwon, J.H. Detection charactenstics of hydrocarbons from irradiated legumes of Korean and Chinese origins. J. Korean Soc. Food Sci. Nutr. 30(5): 770-776 (2001)
  5. Oh, K.N., Kim, K.E. and Yang, J.S. Detection of irradiated beans using the DNA comet assay. J. Korean Soc. Food Sci. Nutr. 29: 843-848 (2000)
  6. Stewart, E.M., Stevenson, M.H. and Gray, R. Use of ESR spec-troscopy for the detection of irradiated Crustacea. J. Sci. Food Agric. 65: 191-197 (1994) https://doi.org/10.1002/jsfa.2740650211
  7. Mckelvey-Martin, V.J., Green, M.H.L., Schmezer, P., Pool-Zobel, B.L., De M$\'{e}$, M. and Collins, A. The single cell electrophoresis assay (comet assay): A European review. Mutat. Res. 288: 47-63 (1993) https://doi.org/10.1016/0027-5107(93)90207-V
  8. Goodman, B.A., McPhail, D.B. and Duthie, D.M.L. Electron spin resonance spectroscopy of some in-adiated food stuffs. J. Sci. Food Agric.47: 101-111 (1989) https://doi.org/10.1002/jsfa.2740470112
  9. Kwon, Y.J., Huh, E.Y., Kwon, J.H. and Byun, M.W. Quarantine status of agncultural products for export and applicadon prospects ofirradiation technology. Food Sci. Ind. 32(2): 80-90 (1999)
  10. Kwon, J.H. Import control of irradiated food. Food Industry 159(1): 61-87 (2001)
  11. Edgar, F.O. de Jesus, Rossi, A.M. and Lopes, R.T. An ESR study on identification of gamma-irradiated kiwi, Papaya and tomato using fruit pulp. Intl. J. Food Sci. Technol. 34: 173-178 (1999) https://doi.org/10.1046/j.1365-2621.1999.00250.x
  12. Korea Agricultural Trade hiformadon homepage. www.kati.co.kr (2001)
  13. Origin. Origin tutorial manual, version 6.0, Microcal Software, Inc., pp. 20-45, Northampton, MA (1999)
  14. Cerda, H., Delincee, H., Haine, H. and RupP, H. The DNA 'Comet assay' as a rapid screening technique to control irradiated food. Mutat. Res. 375: 167-181 (1997) https://doi.org/10.1016/S0027-5107(97)00012-2
  15. Schreiber, G.A., Ziegelmann, B., Quitzsch, G., Helle, N. and B$\ddot o$gl, K.W. Luminescence techniques to identify the treatment of foods by ionizing irradiation. Food Stmcture 12: 385-396 (1993)
  16. Lee, E., Jung, J., Chung, H.W. and Kwon, J.H. Effect of water activity on free radical concentration in detection of irradiated food by ESR spectroscopy. Paper presented at the 64th annual meeting of the Korean Society of Food Science and Technology, Daegu, May 22 (2000)
  17. European Committee for Standard. Detection of irradiated food from which silicate minerals can be isolated. Method by ther-moluminescence. English version of DIN EN 1788 (1997)
  18. Kwon, J.H., Jeong, J., Chung, H.W. and Byun, M.W. Thermolu-minescence characteristics of minerals from irradiated potatoes of different ongind of production. Paper presented at llth Intema-tional Meeting on Radiation Processing. Avignon, France, 25-30 March (2001)
  19. Khan, J.M. and Delinc$'{e}$e, H. Detection of radiation treatment of spices and herbs of asiac origin using thermoluminescence of mineral contaminants. Appl. Radiat. Isot. 46(10): 1071-1075 (1995) https://doi.org/10.1016/0969-8043(95)00193-H
  20. SAS, SAS users guide. SAS Institute Inc., Cary, NC, USA (1986)
  21. Schreiber, G.A., Hoffmann, A., Helle, N. and B$\ddot o$gl, K.W. An interlaboratory trial on the identification of irradiated spices, herbs, and spice-herb mixtures by thermoluminescence analysis. JAOAC Intl. 78: 88-93 (1995)
  22. Kwon, J.H., Chung, H.W. and Byun, M.W. ESR spectroscopy for detecting gamma-irradiated dried vegetables and estimating absorbed doses. Radiat. Phys. Chem. 57: 319-324 (2000) https://doi.org/10.1016/S0969-806X(99)00398-9
  23. IAEA Analytical detection methods for irradiated foods. A review of current literature. IAEA-TECD0C-587, pp. 1-172 (1991)
  24. Jacques, J.R. and Lean-Pierre, L.A. Electron spin resonance iden-tification of irradiated fruits. Radiat. Phys. Chem. 34(6): 891-894 (1989)
  25. Codex Alimentanus Commission, Codex General Standard for Irradiated Foods and Recommended Intemational Code of Prac-tice for the Operation of Radiation Facilides Used for the Treat-ment of Poods. CAC/ VOL, XV, FAO, Rome (1984)
  26. Delince, H. Detection of food treated with ionizing radiation. Trends in Food Sci. Tech. 9: 73-82 (1998) https://doi.org/10.1016/S0924-2244(98)00002-8
  27. Hwang, K.T., Park, J.Y. and Kwon, Y.J. Hydrocarbons detected in irradiated soybeans. Korean J. Food Sci. Technol. 30(3): 517-522 (1998)
  28. Desrosiers, M.F. and Mclaughlin, W.L. Examination of gainma-irradiated fruits and vegetables by electron spin resonance spec-troscopy. Radiat. Phys. Chem. 34: 895-898 (1989)
  29. Koppen, G. and Cerda, H. IdentiScation of low-dose irradiated seeds using the neutral comet assay. Food Sci. Technol. 30: 452-457 (1997)
  30. Chung, H.W. Characterization of irradiated foods by thermolumi-nescence and electron spin resonance measurements for their identification. Ph.D. thesis, Kyungpook National Univ., Daegu, Korea (2000)
  31. Autio, T. and Pmnioja, S. Identification of irradiated foods the thermoluminescence of mineral contamination. Z. Sebensm. Unters. Forsch. 191: 177-180 (1990) https://doi.org/10.1007/BF01197616