Effect of Porphyran isolated from Laver, Porphyra yezoensis, on Liver Lipid Peroxidation in Hyperlipidemic Rats and on Immunological Functions in Mice

김 다당류 porphyran의 급이가 흰쥐의 혈청과 간의 효소활성 및 마우스의 면역에 미치는 영향

  • Jung, Kyoo-Jin (Department of Marine Food Industry, Provincial College of Namdo) ;
  • Jung, Bok-Mi (Department of Food Science and Nutrition, Yosu National University) ;
  • Kim, Seon-Bong (Department of Food and Biotechnology, Pukyong National University)
  • 정규진 (남도대학 해양식품산업과) ;
  • 정복미 (여수대학교 식품영양학과) ;
  • 김선봉 (부경대학교 식품공학과)
  • Published : 2002.04.01


This study was carried out to investigate the effect of porphyran on enzyme activity in rats and immunity in mice. Animals were divided into 5 groups, and were given porphyran diet for 4 weeks. Porphyran was extracted from Porphyra yezoensis: Diet groups were normal diet, control diet fed high fat, cholesterol and sodium cholate, control and 1% porphyran diet (1% PD), control and 5% porphyran diet (5% PD), control and 10% of porphyran diet (10% PD). Also Balb/c female mouse were injected i.p. with porphyran extract every other day for 20 days at levels of 1%, 2% and 5%. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activities were lower in the porphyran diet group than those in control group. Superoxide dismutase and catalase activities in liver homogenates were reduced in porphyran diet group compared to those of control group. Also, the level of liver thiobarbituric acid reactive substance (TBARS) was lower in porphyran group than that of control group. Porphyran increased IL-1 production in a dose-dependent manner, however, interleukine-2 production was reduced as the amount of porphyran increases. These results showed that supplementation of porphyran lowered antioxidant enzyme activities and has possibility of modulating immunological function.


porphyran;antioxidation;immunological function


  1. Park, J.H., Kang, K.C., Baek, S.B., Lee, Y.H. and Rhee, K.S.Separation of antioxidant compounds from edible marine algae.Korean J. Food Sci. Technol. 23: 256-261 (1991)
  2. Cho, K.J., Lee, Y.S. and Ryu, B.H. Antitumor effect and immu-nological activity of seaweeds toward sarcoma-l8O. Bull. Korean Fish. Soc. 23: 345-352 (1990)
  3. Stefan, M. and Gudrun, M. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 469-474 (1974)
  4. Cohen, G., Dembiec, D. and Marcus, J. Measurement of catalase activity in tissue extracts. Agric. Biol. Chem. 34: 30-38 (1970)
  5. Meydani, S.N. Modulation of cytokine production by dietary polyunsaturated fatty acids. P.S.E.B.M. 200: 189-191 (1992)
  6. Devi, M.A. and Das, N.P. Antiproliferlative effect of polyunsatu-rated fatty acid and interleukin-2 on normal and abnormal human lymphocytes. Res. Articles 50: 489-492 (1994)
  7. Reitman, S. and Frankel, S. A colorimetric method for the deter-mination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 28: 56-63 (1957) https://doi.org/10.1093/ajcp/28.1.56
  8. Noda, H., Amano, K. Arashima. Antitumour activity of polysac-charides and lipids from marine algae. Nippon Suisan Gakkaishi, 55(7): 1265-1271 (1989b) https://doi.org/10.2331/suisan.55.1265
  9. Calder, P.C., Bond, J.A. Bevan, S.J. and Newsholeme, E.A.Unsaturated fatty acids inhibit interleukin-2 production by con-canavalin A-stimulated lymphocytes. Proc. Nutr. Soc. 50: 171-175(1991)
  10. 汁啓介 ,森文平 . 食物纖維の科學 , pp. 60-73.高分子水溶性食物纖維 . 朝倉書店 . 日 本 (1997)
  11. Yagoob, P. and Carder, P.C. The effect of fatty acids on lympho-cyte functions. Int. J. Biochem. 12: 1705-1709 (1993)
  12. Jung, K.J., Jung, B.M. and Kim, S.B. Effect of porphyran iso-lated from Laver, Porphyra yezoensis, on lipid metabolism in hyperlipidemic and hypercholesterolemic rats. Korean J. Food Sci.Technol 33: 633-640 (2001)
  13. Ministry of Maritime Affairs & Fisheries. Statistical year book of maritime affairs and fisheries, pp. 1169-1171 (2000)
  14. Shah, S.V., Cruz, F.C., and Baricos, W.H. NADPH-induced chemiluminescence and lipid peroxidation on kidney microsomers. Kidney Int. 23: 691-698 (1983) https://doi.org/10.1038/ki.1983.80
  15. Meydani, S.N. Dietary modulation of cytokine production and biological functions. Nut. Rev. 48: 361-364 (1990) https://doi.org/10.1111/j.1753-4887.1990.tb02880.x
  16. 吉澤康子 , 野村和代 . 紅藻由來免疫賦浴 多糖に關する硏究 .食品工業 37: 25-30 (1994)
  17. Nishide, E., Ohno, M., Anzai, H. and Uchida, N. Extraction of porphyran from Porphyra yezoensis ueda F. narawaensis miura. Nippon Suisan Gakkaishi 54: 2189-2194 (1988) https://doi.org/10.2331/suisan.54.2189
  18. Kaneda, T. and Ando, H. Component lipids of purple laver and their antioxygenic activity. Proc. Int. Seaweed Symp. 7: 553-559(1971)
  19. Jo, K.S., Do, J.R. and Koo, J.G. Pretreatment conditions of Por-phyra yezoensis, Undaria pinnatifida for functional algae-tea. J. Korean Soc. Food Sci. Nutr., 27: 275-280 (1998)
  20. Yoshizawa, Y, Ametani, A., Tsunehiro, J., Nomura, K., Itoh, M.,Fukui, F. and Kaminogawa, S. Macrophage stimulation activity of the polysccharide fraction from a marine algae (Porphyra yezoen-sis): stracture-function relationships and improved solubility. Bio-sci. Biotechnol. Biochem. 59: 1933-1937(1995) https://doi.org/10.1271/bbb.59.1933
  21. Park, Y.B., Kim, I.S., Yoo, S.J., Ahn, J.K., Lee, T.G., Park, D.C.and Kim, S.B. Elucidation of anti-tumor initiator and promoter derived from seaweed-2: Investigation of seaweed extracts Sup-pressing mutagenic activity of PhIP and MeIQx. J. Korean Fish. Soc. 31: 581-586 (1998)
  22. Koo, J.G. and Park, J.H. Chemical and gelling properties of alkali-modified porphyran. J. Korean Fish. Soc. 32: 271-275 (1999)
  23. Lee, B.H., Choi, B.W., Chun, J.H. and Yu, B.S. Extraction of water soluble antioxidants from seaweeds. J. of Korean Ind. & Eng. Chem. 7: 1069 - 1077 (1996)
  24. Lokesh, B.R., Sayer, T.J. and Kinsella, J.E. Interleukin-1 and tumor necrosis factor synthesis by mouse peritoneal macrophage is enhanced by dietary n-3 polyunsaturated fatty acid. Immunol.Letter 23: 281-284 (1989)
  25. Yoshizawa, Y, Enomoto, A., Todoh, H., Ametani, A. and Kami-nogawa, S. Activation of murine macrophages by polysaccharide fractions marine algae (Porphyra yezoensis). Biosci. Biotechnol. Biochem. 57: 1862-1868 (1993) https://doi.org/10.1271/bbb.57.1862