Cholesterol-Lowering Effects in Rat Liver Fed Barley and ${\beta}-Glucan-enriched$ Barley Fraction with Cholesterol

보리 및 보리 ${\beta}-glucan$ 농축획분 섭취에 의한 흰쥐의 간 콜레스테롤 저하효과

  • 김성란 (한국식품개발연구원 식품가공연구본부) ;
  • 석호문 (한국식품개발연구원 식품가공연구본부) ;
  • 최희돈 (한국식품개발연구원 식품가공연구본부) ;
  • 박용곤 (한국식품개발연구원 식품가공연구본부)
  • Published : 2002.04.01


Hypocholesterolemic effects in rats of two hull-less barleys, waxy and nonwaxy-starch genotype, were evaluated by analyzing liver cholesterol and triglycerides. A group fed cellulose control and fiber-free exhibited significant liver lipid accumulation by morphologically observation. In barley fed group, however, lipid disposition in liver was effectively suppressed. The concentrations of cholesterol and triglyceride in liver were significantly reduced in rat fed 5 and 10% fiber diets containing barley flour and ${\beta}-glucan-enriched$ fraction. ${\beta}-Glucan-enriched$ barley fraction as a fiber source showed more cholesterol-lowering than barley flour with cellulose, and this effect of ${\beta}-glucan$ from two hull-less barleys was similar. Liver cholesterol and triglyceride in cellulose control group were 14.5 mg/g and 46.1 mg/g, respectively, while those of barley and ${\beta}-glucan$ fed groups were $5.1{\sim}8.2\;mg/g$ and $20.1{\sim}31.3\;mg/g$, respectively.


barley;${\beta}-glucan-enriched\;fraction$;liver cholesterol;liver triglyceride


  1. Newman, R.K., Lewis, S.E., Newman, C.W., Boik, R.J. and Pamage, R.T. Hypocholesterolemic effect of barley foods on healthy men. Nutr. Rep. Int. 39:749-760 (1989)
  2. Newman, R.K., Newman, C.W. and Graham, H. The hypocholes-terolemic function of barley $\beta$-glucans. Cereal Foods World 34:883-886(1989)
  3. Fedel J.G., Newman, R.K., Newman, C.W. and Barnes, A.E Hypocholesterolemic effects of $\beta$-glucans in different barley diets fed to broiler chicks. Nutr. Rep. Int. 35:1049-1058 (1987)
  4. Klopfenstein, C.F. The role of cereal beta-glucans in nutrition and human health. Cereal Foods World 33:865-869 (1988)
  5. Jackson, K.A., Suter, D.A.I. and Topping, D.L. Oat bran, barley and malted barley lower plasma cholesterol relative to wheat bran but differ in their effects on liver cholesterol in rats fed diets with and without cholesterol. J. Nutr. 124:1678-1684 (1994)
  6. Yang, J.L., Suh, M.J. and Song, Y.S. Effects of dietary fibers on cholesterol metabolism in cholesterol-fed rats. J. Korean Soc.Food Nutr. 25: 392-398 (1996)
  7. Folch, J., Less, M. and Stanley, G.H. A simple method for the isolation and purification of total lipids from animal tissues. J.Biol. Chem. 226:497 -569 (1957)
  8. Seog, H.M., Kim, J.S., Hong, H.D., Kim, S.S. and Kim, K.T. Effect of parboiling on the physicochemical properties of imma-ture barley kernels. J. Korean Agric. Chem. Soc. 36:456-462(1993)
  9. Cara, L., Dubois, C., Borel, P, Armand, M., Senft, M., Portugal,H., Pauli, A.M., Bernard, P.M. and Lairon, D. Effect of oat bran, rice bran, wheat flour, and wheat germ on postprandial lipemia in healthy adults. Am. J. Clin. Nutr. 55:81-88 (1992)
  10. A.O.A.C. Official Methods of Analysis, 15th ed., Association of Official Chemists, Washington, DC, USA (1990)
  11. Anderson, J.W. and Bridges, S.R. Plant fiber metabolites after hepatic glucose and lipid metabolism. Diabetes 30(supp1):133-139(1981)
  12. Marlett, J.A. Dietary fiber content and effect of processing on two barley varieties. Cereal Foods World 36:576-578 (1991)
  13. Ikegami, S., Tsuchihashi, F., Harada, H., Tsuchihashi, N.,Nishide, E. and Innami, S. Effect of viscous indigestible polysac-charides on pancreatic-biliary secretion and digestive organs in rats. J. Nutr. 120:353-360 (1990)
  14. Nishina, P.M., Schneeman, B.O. and Freedland, R.A. Effects of dietary fibers on nonfasting plasma lipoprotein and apolipoprotein levels in rats. J. Nutr. 121:431-437 (1991)
  15. Kang, H.J. and Song, Y.S. Dietary fiber and cholesterol metabo-lism. J. Korean Soc. Food Sci. Nutr. 26:358-369 (1997)
  16. Seog, H.M., Kim, S.R., Choi, H.D. and Kim, H.M. c Korean J. Food Sci. Tech-nol. Submitted (2002)
  17. Qureishi, A.A., Burger, W.C., Pederson, M.D. and Elson, C.E. The structure of an inhibitor of cholesterol biosynthesis isolated from barley. J. Biol. Chem. 261:10544-10550 (1986)
  18. Anderson, J.W., Jones, A.E. and Riddle-Mason, S. Ten different dietary fibers have significantly different effects on serum and liver lipids of cholesterol-fed rats. J. Nutr. 124:78-83 (1994)
  19. Miettinen, T.A. Dietary fiber and lipids. Am. J. Clin. Nutr. 45:1237-1242(1987)
  20. Lee, Y.T., Seog, H.M. and Cho, M.K, $\beta$-Glucan enrichment from pearled barley and milled barley fractions. Korean J. Food Sci.Technol. 29:888-894 (1997)
  21. Vahouny, G.V, Roy, T., Gallo, L.L., Story, J.A., Kritchevsky, D.and Cassidy, M.M. Dietary fiber III. Effect of chronic intake on cholesterol absorption and metabolism in the rat. Am. J. Clin. Nutr. 33:2182-2188 (1980)