Characterization of the Antagonistic Activity against Lactobacillus plantarum and Induction of Bacteriocin Production

김치로부터 Lactobacillus plantarum 생육저해 박테리오신 생산균주의 분리 및 박테리오신 생산의 유도효과

  • Yang, Eun-Ju (Department of Food and Nutrition, Chosun university) ;
  • Chang, Ji-Yoon (Department of Food and Nutrition, Chosun university) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, Seoul National University) ;
  • Kim, Jeong-Hwan (Department of Food Science and Technology, Gyeongsang National University) ;
  • Chung, Dae-Kyun (Department of Genetic Engineering, Kyung Hee University) ;
  • Lee, Jong-Hoon (Department of Food and Biotechnology, Kyunggi University) ;
  • Chang, Hae-Choon (Department of Food and Nutrition, Chosun university)
  • Published : 2002.04.01

Abstract

A new bacteriocin producing lactic acid bacteria having antagonistic activity against Lactobacillus plantarum, was isolated from Kimchi. It was identified as Leuconostoc mesenteroides, and designated as Leuconostoc mesenteroides B7. The bacteriocin from Leuconostoc mesenteroides B7 named as bacteriocin B7 was stable in the pH range $2.5{\sim}9.5$. Bacteriocin B7 was active over a wide temperature range from $4^{\circ}C$ to $120^{\circ}C$. It was inactivated by proteinase K, trypsin, ${\alpha}-chymotrypsin$, and protease treatments indicating its proteinous nature. Tricine-SDS-PAGE of the purified bacteriocin B7 showed the presence of a single band, having a molecular mass of about 3,500 dalton. Mixed culture of the producer and the indicator, Lb. plantarum KFRI 464 or Lb. delbruekii KFRI 347, increased production of bacteriocin B7. This result suggested the presence of bacteriocin inducing factor in the indicator strain. The inducing factor was localized in cell debris and intracellular faction of the indicator cell, Lb. plantarum KFRI 464. Treatment of the inducing factor with proteinase K destroyed inducing activity. This result strongly suggested that the inducing factor is a protein.

Keywords

Leuconostoc mesenteroides;Lactobacillus plantarum;bacteriocin inducing factor;Kimchi

References

  1. Daba, H.S., Pandian, J.F., Gossenelin, R.E., Simard, J.H. and Lac-roix, C. Detection and activity of a bacteriocin produced by Leu-conostoc mesenteroides. Appl. Environ. Microbiol. 57: 3450-3455(1991)
  2. Eijsink, V.G.H., Bruberg, M.B., Middelhoven, P.H. and Nes, I.F.Induction of bacteriocin production in Lactobacillus sake by a secreted peptide. J. Bacteriol. 178: 2232-2237 (1996) https://doi.org/10.1128/jb.178.8.2232-2237.1996
  3. So, M.H. and Kim, Y.B. Identification of psychrotrophic lactic acid bacteria isolated from Kimchi. Korean J. Food Sci. Technol.27(4): 495-505 (1995)
  4. Rammelsberg, M. and Radler, F. Antibacterial polypeptide of Lac-tobacillus species. J. Appl. Bacteriol. 69: 177-184 (1990) https://doi.org/10.1111/j.1365-2672.1990.tb01507.x
  5. Kim, O.M., Kim, M.K., Lee, K.R. and Kim, S.D. Selective serial antimicrobial effects of spice extracts against Lactobacillus plan-tarum and Leuconostoc mesenteroides isolated from Kimchi.Korean c 26(5): 373-378 (1998)
  6. Kim, S.I., Kim, I.C. and Chang, H.C. Isolation and identification of antimicrobial agent producing microorganisms and sensitive strain from soil. J. Korean Soc. Food Sci. Nutr. 28(3): 526-553 (1999)
  7. Schagger, H. and von Jagow, G. Tncin-sodium dodecyl sulfate-polyacryamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368-379(1987) https://doi.org/10.1016/0003-2697(87)90587-2
  8. Daschel, M.A., Mckenney, M.C. and McDonald, L.C. Bacterio-cidal activity of Lactobacillus plantarum C-ll. Food Microbiol.7: 91-98 (1990) https://doi.org/10.1016/0740-0020(90)90014-9
  9. Diep, D.B., Havarstein, L.S. and Nes, I.F. A bacteriocin-like pep-tide induces bacteriocin synthesis in L. plantarum Cll. Mol. Microbiol. 18: 631-639 (1995) https://doi.org/10.1111/j.1365-2958.1995.mmi_18040631.x
  10. Saucier, L., Poon, A. and Stiles, M.E. Induction of bacteriocin in Cartobacterium piscicola LV17. J. Appl. Bacteriol. 78: 684-690(1995) https://doi.org/10.1111/j.1365-2672.1995.tb03116.x
  11. Barefoot, S.F, Chen, Y-R., Bodine, T. A., Shearer, M. Y. and Hughes, M. D. Identification and purification of a protein that induces production of the Lactobacillus acidophilus bactenocin Lactacin B. Appl. Microbiol. 60: 3522-3528 (1994)
  12. Piard. J.C., Delorme, F, Giraffa, G., Commissaire, J. and Des-mazeud, M. Evidence for a bacteriocin produced by Lactococcus lactis CNRZ 481. Neth. Milk Dairy J. 44: 143-158 (1990)
  13. Kim, M.H., Oh, S.W., Hong, S.P. and Yoon S.K. Microbiology/fermentation: antimicrobial characteristics of chitosan and chito-san oligosaccharides on the microorganism related to Kimchi. Korean J. Food Sci. Technol. 30(6): 1439-1447 (1998)
  14. Tagg, J.R. and Mcgiven, A.R. Assay system for bacteriocin. Appl. Microbiol. 21:943 (1971)
  15. Mathieu, F, Suwandhi, I.S.N., Rekhif, J.F. and Lefebvre, G.Mesenterocin 52, a bacteriocin produced by Leuconostoc mesenteroides sp. mesenteroides FR52. J. Appl. Bacteriol. 74: 372-379(1993) https://doi.org/10.1111/j.1365-2672.1993.tb05141.x
  16. Quadri, L.E.N., Kleerebezeem, M., Kuipers, O.R, Roy, K.L.,Veras, J.C. and Stiles, M.E. Characterization of a locus from Car-nobacterium piscicola LV 1713 involved in bacteriocin production and immunity: evidence for global inducer-mediated transcrip-tional regulation. J. Bacteriol. 179: 6163-6179 (1997) https://doi.org/10.1128/jb.179.19.6163-6171.1997
  17. Axelsson, L. and Askild, 0. The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb 706. J. Bacteriol. 177: 2125-2137 (1995) https://doi.org/10.1128/jb.177.8.2125-2137.1995
  18. Lee, C.W., Ko, C.Y. and Ha, D.M. Microfloral of the lactic acid bacteria during Kimchi fermentation and identification of the iso-lates. Korean J. Appl. Microbiol. Biotechnol. 20(10): 102-109 (1992)
  19. Liu, W. and Hansen, J.N. Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lac-tis. Appl. Environ. Microbiol. 56: 2551-2558(1990)
  20. Kang, S.M., Yung, W.S., Kim, Y.C., Joung, E.Y. and Han, Y.G. Strain improvement of Leuconostoc mesenteroides for Kimchi fer-mentation and effect of starter. Korean J. Appl. Microbiol. Bio-technol. 23(4): 461-471 (1995)