Characterization of the Antagonistic Activity against Lactobacillus plantarum and Induction of Bacteriocin Production

김치로부터 Lactobacillus plantarum 생육저해 박테리오신 생산균주의 분리 및 박테리오신 생산의 유도효과

  • Yang, Eun-Ju (Department of Food and Nutrition, Chosun university) ;
  • Chang, Ji-Yoon (Department of Food and Nutrition, Chosun university) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, Seoul National University) ;
  • Kim, Jeong-Hwan (Department of Food Science and Technology, Gyeongsang National University) ;
  • Chung, Dae-Kyun (Department of Genetic Engineering, Kyung Hee University) ;
  • Lee, Jong-Hoon (Department of Food and Biotechnology, Kyunggi University) ;
  • Chang, Hae-Choon (Department of Food and Nutrition, Chosun university)
  • Published : 2002.04.01


A new bacteriocin producing lactic acid bacteria having antagonistic activity against Lactobacillus plantarum, was isolated from Kimchi. It was identified as Leuconostoc mesenteroides, and designated as Leuconostoc mesenteroides B7. The bacteriocin from Leuconostoc mesenteroides B7 named as bacteriocin B7 was stable in the pH range $2.5{\sim}9.5$. Bacteriocin B7 was active over a wide temperature range from $4^{\circ}C$ to $120^{\circ}C$. It was inactivated by proteinase K, trypsin, ${\alpha}-chymotrypsin$, and protease treatments indicating its proteinous nature. Tricine-SDS-PAGE of the purified bacteriocin B7 showed the presence of a single band, having a molecular mass of about 3,500 dalton. Mixed culture of the producer and the indicator, Lb. plantarum KFRI 464 or Lb. delbruekii KFRI 347, increased production of bacteriocin B7. This result suggested the presence of bacteriocin inducing factor in the indicator strain. The inducing factor was localized in cell debris and intracellular faction of the indicator cell, Lb. plantarum KFRI 464. Treatment of the inducing factor with proteinase K destroyed inducing activity. This result strongly suggested that the inducing factor is a protein.


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