Anticarcinogenic Effects of Allium tuberosum on Human Cancer Cells

부추 추출 성분의 항발암 효과 연구

  • Park, Yun-Ja (Department of Food and Nutrition, Silla University) ;
  • Kim, Mi-Hyang (Department of Food and Nutrition, Silla University) ;
  • Bae, Song-Ja (Department of Food and Nutrition, Silla University)
  • 박윤자 (신라대학교 식품영양학과) ;
  • 김미향 (신라대학교 식품영양학과) ;
  • 배송자 (신라대학교 식품영양학과)
  • Published : 2002.08.01

Abstract

The anticarcinogenic effects of various food components have received much attention in recent years. However mechanism of anticarcinogens in food materials on cancer cells have rarely been investigated. This study was performed to investigate the effects on the cytotoxicity and quinone reductase (QR) activity of Allium tuberusum (AT) on the human cancer cells. The six partition layers which are methanol (ATM), hexane (ATMH), ethylether (ATMEE), ethylacetate (ATMEA), butaonl (ATMB) and aqueous (ATMA) of Allium tuberusum were screened for their cytotoxic effects on HepG2, MCF-7, HeLa and SK-N-MC cells by the MTT assay. Among the six partition layers, ATMEE had the strongest cytotoxic effect at concentration of $150\;{\mu}g/mL$ which resulted over 95% on HepG2, HeLa, MCF-7 and SK-N-MC cell lines. The ATMEA also showed significant cytotoxic effect on HepG2 and SK-N-MC cell lines. The ATMB showed the highest induction activity of QR on HepG2 cells among the other partition layers. QR activity of HepG2 cells, grown in the presence of ATMB at the concentration of $50\;{\mu}g/mL$, was increased by 3.9 times, compared to the control value of 1.0. Based on these results, the ATMEE and ATMB may have potentially anticarcinogenic and chemopreventive activities.

References

  1. Zhang, Y., Talalay, R., Cho, C.G. and Posner, G.H. An anticarcinogenic protective enzyme from broccoli. Porc. Natl. Acad. Sci., 89: 2399-2403 (1992) https://doi.org/10.1073/pnas.89.6.2399
  2. Oh, H.S. and Ham, S.S. Antimutagenic effect of enzymatic browning reaction products of polyphenol compounds by polyphenol oxidase derived from mushroom. J. Korean Soc. Food Nutr., 21, 341-346 (1992)
  3. Hellman, K., Phil, D. and Carter, S.K. Fundamental of cancer chemother. p. 64. McGraw Hill Book co., New York, USA (1987)
  4. Giampietri, A. Drug-mediated increase of tumor immunogenicity in vivo for a new approach to experimental cancer immunotherapy. Cancer Res. 41: 681-687 (1981)
  5. Budd, G.T., Osgood, B., Burna, B., Boyett, J.M., Finke, J., Mden-drop, S.V., Murth, S., Bukoski, R.M. Phas I clinical trial Toxicity and immunologic effects, Cancer Res,. 49: 6432 (1989)
  6. Ha, Y.L. and Michael, W.P. Naturally occuring novel auticarcinogenes: Conjugated dienoic derivatices of linoleic acid (CLA) (in Korean). J. Korean Soc. Food Nutr., 20: 401-407 (1991)
  7. Chihara G, Hamuro J, Maeda Y, Arai Y, Fukuoka Y. Fructionation and purification of the polysaccharides with marked antitumor activity, especially Lentinarr, from Lentinusedodes sing. Cancer Res. 30: 2276-2278 (1970)
  8. Choi, O.J. Component and Using of Medical Plants, p. 791 Ilwoulseogak, Seoul (1999)
  9. The Korean Society of Nutridon. Recommended Dietary Allow-ances for Koreans., 7th revision (2000)
  10. Sohn, H.E., Lee, J.Y, Kim, D.C. and Hwang, W.I. Enhancement of Anticancer Activity by Combination of Garlic (Allium Sati-vum) Extract and vitamin C. J. Korean Soc. Food Sci. Nutr. 30: 372-376 (2001)
  11. Sheo, H.J. The Antibacteril Action of Garlic, Onion, Ginger and Red Pepper Juice. J. Korean Soc. Food Sci. Nutr. 28: 94-99(1999)
  12. Kim, S.O. and Lee, M.Y. Effects of Ethylacetate Fracdon of Onion on Lipid Metabolism in High Cholesterol-Fed Rats. J. Korean Soc. Food Sci. Nutr. 30: 673-678 (2001)
  13. Hong, S.A. and Wang, S.G. Effects of Korean Leek and Dietary Fat on Plasma Lipids and Platelet Aggregation in Hypercholesterolemic Rats. The Korean Nuthtion Society. 33: 374-385 (2000)
  14. Park, E.R., Jo, J.O., Kim, S.M., Lee, M.Y. and Kim, K.S. Volatile Flavor Components of Leek (Allium tuberosum Rotter). J. Korean Soc. Food Sci. Nutr. 27: 563-567 (1998)
  15. Kwak, Y.J., Jun, H.J., Lee, M.J., Kwon, T.W. and Kim, J.S. Modulation of Anticarcinogenic Enzyme and Plasma Testosterone Level in Male Fed Leek supplemented Diet. J. Korean Soc. Food Sci. Nutr. 27: 968-972 (1998)
  16. Michael C. Alley, Dominic A Scudiero, Anne Monks, Miriam L. Hursey, Maciej J. Czerwinski, Donald L. Fine, Betty J. Abbortt, Joseph G. Mayo, Robert H. Shoemaker and Michael R. Boyd Feasibility of Drug Screening with Panels of Human Tumor Cell lines Using a Microculture Tetrazolium Assay. Cancer Res. 48: 589-601 (1988)
  17. Carmichael, J., De Graff, W.G., Gazder, A.F., Minna, J.D. and Mitchell, J.B. Evalution of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942 (1987)
  18. Prochaska, H.J. and Santamaria, A.B. Direct measurement of NAD(P)H: Quinone reductase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme in ducers. Anal. Biochem. 169: 328-336 (1988) https://doi.org/10.1016/0003-2697(88)90292-8
  19. Fischer, S.M., Leyton, L.J., Lee, M.L., Locnislcar, M., Belury, M.A. and Maldve, R.E. Differential effects of dietary linoleic acid on mouse skin-tumor promotion and mammary carcinogenesis. Cancer Res. 52: 2049-2056 (1992)
  20. Han, E.J., Roh, S.B. and Bae, S.J. Cytotoxicity of Daucus carota L. on verious cancer cells. J. Korean Soc. Food Sci. Nutr. 29: 153-160(2000)
  21. Park, Y.J., Kim, M.H. and Bae, S.J. Enhancement of anticarcinogenic effect by combination of Sedum sarmentosum bunge with Platycodon grandiflorum a. extracts. J. Korean Soc. Food Sci Nutr. 3l: 136-142(2002) https://doi.org/10.3746/jkfn.2002.31.1.136
  22. Godon, G.B., Prochaska, H.J. and Yang, L.Y. Induction of NAD(P)H: quinone reductase on human peripheral blood lymphocytes. Carcinogenesis 12: 2393-2399 (1991) https://doi.org/10.1093/carcin/12.12.2393
  23. Han, E.J., Roh, S.B. and Bae, S.J. Effects of quinone reductase induction and cytotoxicity of the Angelica radix extracts. J. Korean Soc. Food Sci. Nutr. 29: 147-152 (2000)
  24. Hong, E.Y., Kang, H.J. and Kim, J.S. Fractionation of anticarcinogenic enzyme mducers from Rosted perilla. J. Korean Soc. Food Sci. Nutr. 26: 193-197 (1997)
  25. Park, Y.J., Kim, M.H. and Bae, S.J. Enhancement of anticarcinogenic effect by combination of Lycii fructus with vitamin C. J. Korean Soc. Pood Sci. Nutr. 31: 143-148 (2002) https://doi.org/10.3746/jkfn.2002.31.1.143
  26. Shim, S.M., Choi, S.W. and Bae, S.J. Effects of Punica grantum L. Practions on Quinone reductase induction and Growth inhibition on several cancer cells. J. Korean Soc. Food Sci. Nutr. 30: 80-85 (2001)