Effect of γ-Irradiation on the Molecular Properties of Myoglobin

  • Lee, Yong-Woo (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
  • Published : 2002.11.30


To elucidate the effect of gamma-irradiation on the molecular properties of myoglobin, the secondary and tertiary structures, as well as the molecular weight size of the protein, were examined after irradiation at various irradiation doses. Gamma-irradiation of myoglobin solutions caused the disruption of the ordered structure of the protein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains. A SDS-PAGE study indicated that irradiation caused initial fragmentation of the proteins and subsequent aggregation, due to cross-linking of the protein molecules. The effect of irradiation on the protein was more significant at lower protein concentrations. Ascorbic acid protected against the degradation and aggregation of proteins by scavenging oxygen radicals that are produced by irradiation. A circular dichroism study showed that an increase of the irradiation decreased the a-helical content of myoglobin with a concurrent increase of the aperiodic structure content. Fluorescence spectroscopy indicated that irradiation increased the emission intensity that was excited at 280 nm.


  1. Chen, Y., Yang, J. and Chau, K. (1974) Determination of the helix and beta form of proteins in aqueous solution by circular dichroism. Biochemistry 13, 3350-3359.
  2. Cho, Y. and Song, K. B. (1997) Effect of chaotropic salt on the secondary structure of pig skin gelatin. Biosci. Biotech. Biochem. 61, 1194-1195.
  3. Cho, Y. and Song, K. B. (2000) Effect of g-irradiation on the molecular properties of BSA and b-Iactoglobulin. J. Biochem. Mol. Biol. 33, 133-137.
  4. Davies, K. J. A. and Delsignore, M. E. (1987) Protein damage and degradation by oxygen radicals III. Modification of secondary structure and tertiary structure. J. BioI. Chem. 262, 9908-9913.
  5. Filali-Mouhim, A., Audette, M., St-Louis, M., Thauvette, L., Denoroy, L., Penin, E., Chen, X ., Rouleau, N., Le Caer, J. P., Rossier, J., Potier, M. and Le Maire, M. (1997) Lysozyme fragmentation induced by g-radiolysis. Int. J. Radial. BioI. 72, 63-70.
  6. Garrison, W. M. (1987) Reaction mechanisms in the radiolysis of peptides, polypeptides. and proteins. Chem. Rev. 87, 381-398.
  7. Kempner, E. S. (1993) Damage to proteins due to the direct action of ionizing radiation. Quart. Rev. Biophys. 26, 27-48.
  8. Krumhar, K. C. and Beny, J. W. (1990) Effect of antioxidant and conditions on solubility of irradiated food proteins in aqueous solutions. J. Food Sci. 55, 1127-1132.
  9. Kume, T. and Matsuda, T. (1995) Changes in structural and antigenic properties of proteins by radiation. Radial. Phys. Chem. 46, 225-231.
  10. Laemlli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680- 685.
  11. Lee, M. J. and Song, K. B. (1997) Purification of streptodornase from Streptococcus equisimilis and its DNA-induced conformational change. Biochem. Biophys. Res. Commun. 230, 13-15.
  12. Le Maire, M., Thauvette, L., De Foresta, B., Viel, A., Beauregard, G. and Potier, M. Effects of ionizing radiations on proteins. Biochem. J. 267, 431-439.
  13. Li, C., Nishiyama, K. and Taniguchi, I. (2000) Electrochemical and spectroelectrochemical studies on cobalt myoglobin. Electrochim. Acta 45, 2883-2888,
  14. Moczygemba, C., Guidry, J. and Wittung-Stafshede, P. (2000) Heme orientation affects holo-myoglobin folding and unfolding Kinetics, FEBS Lett. 470, 203-206.
  15. Moon, S. and Song, K. B. (2001) Effect of gamma-irradiation on the molecular properties of ovalbumin and ovomucoid and protection by ascorbic acid. Food Chem. 74, 479-483.
  16. Puchala, M. and Schessler, H. (1993) Oxygen effect in the radiolysis of proteins. Int. J. Radiat. Biol. 64, 149-156.
  17. SchuessJer, H. and Schilling, K. (1984) Oxygen effect in the radiotysis of proteins. Int. J. Radiat. Biol. 45, 267-281.
  18. Stellwagen, E. (1978) Haem exposure as the determinate of oxidation-reduction potential of haem proteins. Nature 275, 73- 74.
  19. Takeda, K., Wada, A., Yamamoto, K., Hachiya, K. and Batra, P. P. (1988) Secondary structure change of myoglobin induced by sodium dodecyl sulfate and its kinetic aspects. J. Colloid interface Sci. 125, 307-313.
  20. Wolff, S. P., Garner, A. and Dean, R. T. (1986) Free radicals, lipids and protein degradation. Trends Biochem. Sci. 11, 27-31.
  21. Yang, J. T., Wu, C. and Martinez, H. M. (1986) Calculation of protein conformation from circular dichroism. Methods Enzymol. 130, 208-269.
  22. Yoshimura, T., Matsuno, K., Miyazaki, T., Suzuki, K. and Watanabe, M. (1993) Electron spin resonance studies of free radicals in gamma-irradiated golden hamster embryo cells: radical formation at 77 and 295K, and radioprotective effects of vitamin C at 295K. Radiat. Res. 136, 361-365.

Cited by

  1. Effect of γ-irradiation on the physicochemical properties of soy protein isolate films vol.72, pp.1, 2005,
  2. A potential practical approach to reduce Ara h 6 allergenicity by gamma irradiation vol.136, pp.3-4, 2013,
  3. In Situ Formation of Protein–Polymer Conjugates through Reversible Addition Fragmentation Chain Transfer Polymerization vol.119, pp.17, 2007,
  4. Production and Characterization of Monoclonal Antibodies against Human Ceruloplasmin vol.38, pp.1, 2005,
  5. Alteration of Seed Storage Protein Composition in Soybean [Glycine max (L.) Merrill] Mutant Lines Induced by γ-Irradiation Mutagenesis vol.59, pp.23, 2011,
  6. Impact of irradiation and thermal processing on the immunoreactivity of shrimp(Penaeus vannamei) proteins vol.87, pp.6, 2007,
  7. Reactivity of prednisolone to gamma radiation in aqueous and organic solutions vol.20, pp.3, 2010,
  8. Effect of Electron Beam Irradiation on Remaining Activity of Lyophilized Acid Phosphatase with Water-Binding and Non-Water–Binding Additives vol.33, pp.7, 2015,
  9. In Situ Formation of Protein–Polymer Conjugates through Reversible Addition Fragmentation Chain Transfer Polymerization vol.46, pp.17, 2007,
  10. Improvement of chaperone activity of 2-Cys peroxiredoxin using electron beam vol.81, pp.8, 2012,
  11. Effect of ionizing radiation on structural and functional attributes of red kidney bean (Phaseolus vulgaris L.) lectin vol.59, pp.1, 2014,
  12. Combined effect of arsenic trioxide and radiation on physical properties of hemoglobin biopolymer vol.7, pp.4, 2014,
  13. Sterilization and protection of protein in combinations of Camellia sinensis green tea extract and gamma irradiation vol.48, pp.3, 2011,
  14. Protective role of l-tyrosine in the sterilization of Ceruloplasmin therapeutic protein by gamma-irradiation vol.71, pp.1-2, 2004,
  15. Effects of electron-beam irradiation on physicochemical properties of starches separated from stored wheat vol.63, pp.3, 2011,
  16. UV radiation sensitivity of bovine serum albumin bound to silver nanoparticles vol.7, pp.4, 2014,
  17. Oxidative changes and desialylation of serum proteins in hyperthyroidism vol.337, pp.1-2, 2003,
  18. High doses of gamma radiation suppress allergic effect induced by food lectin vol.85, 2013,
  19. Generation of Polypeptide-Templated Gold Nanoparticles using Ionizing Radiation vol.29, pp.32, 2013,
  20. Effect of high intensity ultrasound on the allergenicity of shrimp vol.7, pp.4, 2006,
  21. Analysis of sterilization protocols for peptide-modified hydrogels vol.74B, pp.1, 2005,
  22. Reduction of allergenic properties of shrimp (Penaeus Vannamei) allergens by high intensity ultrasound vol.223, pp.5, 2006,
  23. Effect of gamma-irradiation on physico-chemical and functional properties of arrowhead (Sagittaria sagittifolia L.) tuber flour vol.11, 2015,
  24. Mode of bindings of zinc oxide nanoparticles to myoglobin and horseradish peroxidase: A spectroscopic investigations vol.110, pp.2, 2011,
  25. Irradiation of myoglobin by intense, ultrashort laser pulses vol.122, pp.10, 2016,
  26. Effect of γ-irradiation on the molecular properties of bovine serum albumin vol.100, pp.2, 2005,
  27. Low-dose gamma irradiation of food protein increases its allergenicity in a chronic oral challenge vol.51, 2013,
  28. Myoglobin functioning as cytochrome P450 for biosensing of 2,4-dichlorophenol vol.4, pp.10, 2012,
  29. Protein Oxidation in Processed Meat: Mechanisms and Potential Implications on Human Health vol.14, pp.2, 2015,
  30. Effects of low-dose ionizing radiation on α,β-globulins solutions studied by DSC vol.111, pp.3, 2013,
  31. Radiation-induced protein fragmentation and inactivation in liquid and solid aqueous solutions. Role of OH and electrons vol.72, pp.2-3, 2005,
  32. Effect of gamma radiation on the structural and biological properties of Angiotensin II vol.84, pp.11, 2008,
  33. Colour and sarcoplasmic protein evaluation of pork following water bath and ohmic cooking vol.93, pp.4, 2013,
  34. Enhancement of Chaperone Activity of Plant-Specific Thioredoxin through γ-Ray Mediated Conformational Change vol.16, pp.11, 2015,