DOI QR코드

DOI QR Code

Construction of Genetic Microsatellite Maps for Some Chromosomes in Chinese Swine Reference Population

Su, Yuhong;Xiong, Yuanzhu;Zhang, Qin;Liu, Weimin;Jiang, Siwen;Yu, Li;Xia, Xuanyan;Zeng, Rong;Deng, Changyan

  • 투고 : 2001.11.30
  • 심사 : 2002.05.01
  • 발행 : 2002.10.01

초록

In aiming to identify the genes or genetic regions responsible for quantitative traits, a swine reference population had been constructed using three Large White boars and seven Meishan dams as parents. Five $F_1$ males and 23 $F_1$ females were intercrossed to generate 147 $F_2$ offspring. Thirty-one microsatellite markers covering Sus scrofa chromosomes (SSC) 2, 4, 6 and 7 were genotyped for all members. Construction of genetic microsatellite maps was performed using the CRIMAP software package. The lengths of these chromosomes were longer than MARC maps. They were 158.6cM, 180.3cM, 197.3cM and 171.4cM, respectively. A two modified orders of markers were observed for SSC6 and SSC7. The female map on SSC6 was shorter than male map, and the contrary was on SSC 2, 4 and 7.

키워드

Swine;Chromosomes 2;4;6;and 7;Crimap Software;Linkage Maps

참고문헌

  1. Bassam, B. J., G. Caetano-Anolles and P. M. Gresshoff. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196:80-83. https://doi.org/10.1016/0003-2697(91)90120-I
  2. Paszek, A. A., L. B. Schook, C. F. Louis, J. R. Michelson, G. H. Flickinger, J. Murtaugh, J. R. Mendiola, M. A. Janzen, C. W. Beattie, G. A. Rohrer, L. J. Alexander, L. Andersson, H. Ellegren, M. Johansson, P. Mariani, L. Marklund, B. Hoyheim, W. Davies, M. Fredholm, A. L. Archibald and C. S. Haley. 1995. First international workshop on porcine chromosome 6. Anim. Genet. 26:377-401. https://doi.org/10.1111/j.1365-2052.1995.tb02689.x
  3. Paszek, A. A., L. Andersson, P. Mariani, L. Marklund, M. Fredholm, B. Hoyheim, A. L. Archibald, V. H. Nielsen, D. Milan and M. A. M. Groenen. 1997. A consensus linkage map for swine chromosome 7. Anim. Genet. 28:223-229. https://doi.org/10.1111/j.1365-2052.1997.00116.x
  4. Rattink, A. P., D. J. de Koning, M. Faivre, B. Harlizins, J. A. M van Arendonk and M. A. M. Groenen. 2000. Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm. Genome. 11:656-661. https://doi.org/10.1007/s003350010117
  5. Rohrer, G. A., L. J. Alexander, Z. Hu, T. P. L. Smith, J. W. Keele and C. W. Beattie. 1996. A comprehensive map of the porcine genome. Genome Res. 6:371-391. https://doi.org/10.1101/gr.6.5.371
  6. Su, Y. H., R. Zeng, S. W. Jiang, C. Y. Deng and Y. Z. Xiong. 2000. Research on polymorphism of 12 microsatellite markers in reference family by Large White ${\times}$ Meishan pigs. Animal Biotechnology Bulletin. 7:166-170.
  7. Ott, J. 1993. Analysis of Human Genetic Linkage. Revised edition. The Johns Hopkins University Press, Baltimore and London.
  8. Wang, L., T.-P. Yu, C. K. Tuggle, H.-C. Liu and M. F. Rothschild. 1998. A directed search for quantitative trait loci on chromosomes 4 and 7 in pigs. J. Anim. Sci. 76:2560-2567.
  9. Haley, C. S. and S. A. Knott. 1992. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity. 69:315-324.
  10. Ellegren, H., B. Chowdhary, M. Johansson and L. Andersson. 1994. Integrating the porcine physical and linkage map using cosmid-derived markers. Anim. Genet. 25:155-164. https://doi.org/10.1111/j.1365-2052.1994.tb00104.x
  11. Knott, S. A., L. Marklund, C. S. Haley, K. Andersson, W. Davies, H. Ellegren, M. Fredholm, I. Hansson, B. Hoyheim, K. Lundstrom, M. Moller and L. Andersson. 1998. Multiple marker mapping of quantitative trait loci in a cross between outbred wild boar and large white pigs. Genetics. 149:1069-1080.
  12. Mikawa, S., T. Akita, N. Hisamatsu, Y. Inage, Y. Ito, E. Kobayashi, H. Kusumoto, T. Matsumoto, H. Mikami, M. Minezawa, S. Shimanuki, C. Sugiyama, Y. Uchida, Y. Wada, S. Yansi and H.Yasae. 1999. A linkage map of 243 DNA markers in an intercross of Gottingen miniature and Meishan pigs. Anim. Genet. 30:407-417. https://doi.org/10.1046/j.1365-2052.1999.00493.x
  13. Archibald, A. L., C. Haley, J. Brown, S. Couperwhite, H. McQueen, D. Nicholson, W. Coppieters, A. Van de Weghe, A. Stratil, A. Wintero, M. Fredholm, N.. Larsen, V. Nielsen, D. Milan, N. Woloszyn, A. Robic, M. Dalens, J. Riquet, J. Gellin and J. C. Caritez. 1995. The PiGMaP consortium linkage map of the pig (Sus scrofa). Mamm. Genome. 6:157-175. https://doi.org/10.1007/BF00293008
  14. Green, P., K. Falls and S. Crooks. 1990. Documentation for CRIMAP. Version 2.4. Washington University School of Medicine, St. Louis. Missouri.
  15. SAS Institute Inc. 1989. SAS/STAT User's Guide: Version 6. 4th edu. SAS Institute Inc., Cary, North Carolina.
  16. Rohrer, G. A., L. J. Alexander, C. W. Beattie, P. Wilkie, G. H. Flickinger, L. B. Schook, A. A. Paszek, L. Andersson, P. Mariani, L. Marklund, M. Fredholm, B. Hoyheim, A. L. Archibald, V. H. Nielsen, D. Milan and M. A. M. Groenen. 1997. A consensus linkage map for swine chromosome 7. Anim. Genet. 28:223-229. https://doi.org/10.1111/j.1365-2052.1997.00116.x
  17. De Koning, D. J., L. L. G. Janss, A. P. Rattink, P. A. M. van Oers, B. J. de Veries, M. A. M. Groenen, J. J. van der Poel, P. N. de Groot, E. W. (Pim) Brascamp and J. A. M. van Arendonk. 1999. Detection of quantitative trait loci for backfat thickness and intramuscular fat content in pigs (Sus scrofa). Genetics. 152:1679-1690.
  18. Marklund, L., M. J. Moller and B. Hoyheim. 1996. A comprehensive linkage map of the pig based on a wild pig-Large White intercross. Anim. Genet. 27:155-169.
  19. Alexander, L. J., G. A. Rohrer and C. W. Beattie. 1996. Cloning and characterization of 414 polymorphic porcine microsatellites. Anim. Genet. 27:137-148. https://doi.org/10.1111/j.1365-2052.1996.tb00941.x
  20. Andersson, L., C. S. Haley, H. Ellegren, S. A. Knott, M. Johansson, K. Anderss, L. Andersson-Eklund, I. Edfors-lilja, M. Fredholm and I. Hansson. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science. 263:1771-1774. https://doi.org/10.1126/science.8134840

과제정보

연구 과제 주관 기관 : Major State Research Development Program, Science Foundation of Hubei Province