Characteristics of High Acidity Producing Acetic Acid Bacteria Isolated from Industrial Vinegar Fermentation

발효 중인 양초식초에서 분리한 고산도 초산균의 생육특성

  • 박미화 (경상대학교 응용생명과학부) ;
  • 류동규 (경상대학교 응용생명과학부) ;
  • 류충호 (경상대학교 응용생명과학부)
  • Published : 2002.06.01


Acetic acid bacteria have been isolated from running high-acid vinegar fermentation. The color of the isolated colony was beige-yellowish. Isolated cell was rod-shaped, small, pale, absolutely aerobic and gram-negative. Microscopically the cells appeared as non-motile and non-flagellated, preferentially occuring in pairs. The optimum temperature and pH for culture were 30$\^{C}$ and 2.7, respectively. The strain was able to grow in the presence of acetic acid, ethanol and glucose. Ethanol was oxidized to acetic acid which was not oxidized any more. The isolated strain utilized glucose, fructose, maltose, sucrose, mannitol and sorbitol as carbon source. Cellulose formation was not detected on bouillon. The DNA (G +C) content of isolated strain was determined to be 56.2 mol%. The strain isolated from industrial vinegar fermentation was identified as Gluconacetobacter europaeus.


  1. Ha YD, Kim KS. 2000. Civilization history of vinegar. Food Indust Nutr 5: 1-6
  2. Yoon HN. 1998. Simultaneous gas chromatographic analysis of ethanol and acetic acid in vinegar. Kor J Food Sci Technol 30: 1247-1251
  3. Siever M, Teuber M. 1995. The microbiology and taxonomy of Acetobacter europaeus in commercial vinegar production. J Appl Bacteriol Symp Suppl 79: 84S-95S
  4. Kittelmann M, Stamnn WW, Follmann H, Truper HG. 1989. Isolation and classification of acetic acid bacteria from high percentage vinegar fermentation. Appl Microbiol Biotechnol 30: 47-52
  5. Stephan J, Sokollek CH, Walter PH. 1998. Culture and preservation of vinegar bacteria. J Biotechnol 60: 195-206
  6. Entani E, Ohmori S, Masai H, Suzuki KI. 1985. Acetobacter polyoxogenes sp. nov., a new species of an acetic acid bacterium useful for producing vinegar with high acidity. J Gen Appl Microbiol 31: 475-490
  7. Yang HC, Choi DS. 1979. Physiological characteristics of acetic acid bacteria isolated from clover flower vinegar. J Kor Agricul Chem Soc 22: 150-159
  8. Cha YJ, Park DK, Chun HS, Lee BK, Kim KH, Lee SY, Kim SJ. 1994. Isolation and characterization of cellulose producing Acetobacter xylinum KI strain. Kor J Appl Microbio Biotechnol 22: 571-576
  9. Shim KC, Lee KS, Kim DH, Ryu IH, Lee JS. 2001. Studies on the acid tolerance of Acetobacter sp. isolated from persimmon vinegar. Kor J Food Sci Technol 33: 574-581
  10. Hammers WP, Sokollek SJ. 1997. Description of a starter culture preparation for vinegar fermentation. Syst Appl Microbiol 20: 481-491
  11. Chae CM. 1977. Maceration Methods for SEM Obserbation. 'A legal medicine lecture room'. Kyungpook National University. p 40-58
  12. Sievers M, Sellmer S, Teuber M. 1992. Acetobacter europaeus sp. nov., a main component of industrial vinegar fermenters in central europe. Syst Appl Microbiol 15: 386-392
  13. Gosselé F, Swings J, De Ley J. 1980. A rapid, simple and simultaneous detection of 2-keto-, 5-keto- and 2,5-diketogluconic acids by thin-layer chromatography in culture media of acetic acid bacteria. Zentbl Bakteriol Mikrobiol Hyg I Abt Orig C 1: 178-181
  14. Marmur J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208-218
  15. Tamaoka J, Komagata K. 1984. Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 25: 125-128
  16. Kim SJ, Chun HS. 1993. Characterization of a new acidophillic Acetobacter sp. strain HA isolated from Korean traditional fermented vinegar. J Microbiol Biotechnol 3: 108- 114
  17. Chung DH. 1980. Studies on the vinegar fermentation. Chungang University Collection of Thesis 24: 181-196
  18. Lee DS, Ryu IH, Lee KS, Shin YS, Chun SH. 1999. Optimization in the preparation of Aloe vinegar by Acetobacter sp. and inhibitory effect against lipase activity. J Kor Soc Agric Chem Biotechnol 42: 105-110
  19. Schüller G, Hertel C, Hammers WP. 2000. Gluconacetobacter entanii sp. nov., isolated from submerged high-acid industrial vinegar fermentations. Int J Syst Evol Microbiol 50: 2013-2020
  20. Sokollek SJ, Hertel C, Hammers WP. 1998b. Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations. Int J Syst Bacteriol 48: 935-940
  21. De Ley J, Swings J, Gosselé F. 1984. Bergey's Manual of Systematic Bacteriology. The Williams and Wilkins Co., Baltimore. Vol 1, p 257
  22. Yamada Y, Kondo K. 1984. Gluconacetobacter, a new subgenus comprising the acetate-oxidizing acetic acid bacteria with ubiquinone-10 in the genus Acetobacter. J Gen Appl Microbiol 30: 297-303
  23. Seo JH, Jeong YJ, Kim JN, Woo CJ, Yoon SR, Kim TH. 2001. Quality comparison of potato vinegars produced by various Acetobacter bacteria. Kor J Postharvest Sci Technol 8: 60-65
  24. Noh WS. 1978. Studies on the acetic acid fermentation. Kor J Appl Microbiol Bioeng 6: 115-120

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