DOI QR코드

DOI QR Code

Enhancement of Antioxidation Effect of Platycodon grandiflorum with Vitamin C on the DLPC Liposomes

DLPC Liposome에 미치는 도라지 추출성분의 비타민 C 첨가에 의한 항산화력 상승효과

  • 배송자 (신라대학교 식품영양학과) ;
  • 강보영 (신라대학교 식품영양학과) ;
  • 김미향 (신라대학교 식품영양학과)
  • Published : 2002.06.01

Abstract

The effect of antioxidant activity of Platycodon grandiflorum (PG) on the liposomal phospholipid membrane was investigated by spectrophotometry. Membrane oxidation causes damage to the membrane fluidity and permeability. It brings further destruction to the sustenance of biological homeostasis. In addition, it is related to several diseases, aging and carcinogenesis. The sample PG was extracted and fractionated to five different types; butanol (PGMB), ethylacetate (PGMEA), ethylether (PGMEE), hexane (PGMH) and methanol (PGMM). The oxidation indices of PGMEA and PGMEE fractions in oxidized dilinoleoylphosphatidylcholine (DLPC) liposomes had stronger antioxidant activities than that of ${\alpha}$-tocopherol and were similar to antioxidant activities compared with butylated hydroxy toluene (BHT), a well-known potent antioxidant, in oxidized DLPC liposomes. The oxidation indices of PGMM extract, PGMB and PGMH fractions exhibited weak antioxidant activity compared with ${\alpha}$-tocopherol in oxidized DLPC liposomes. The oxidation indiex of PGMEE fractions added with vitamin C showed even strong antioxidant activity in the oxidized DLPC liposomes. The oxidation activity of BHT with vitamin C also proved to be stronger than BHT without vitamin C. Therefore vitamin C evidently helps to improve the effect of antioxidant in DLPC liposomes. These results indicate that potentially bioactive substances in PGMEE fraction has a function as potent antioxidant against phospholipid membrane oxidation.

References

  1. Houslay MD, Stanley KK. 1982. Dynamics of Biological Membrance. John Wiley and Son, New York
  2. Noh OJ, Roh SB, Bae SJ. 2000. Effects of Chrysanthemum coronarium L. on the thermotropic behavior of DPPC lipo-somal Membrane. Korean J Life Science 10: 27-32
  3. Aloia RC. 1983. Membrane Fluidity in Biology. Academic Press, New York
  4. Gray JI. 1978. Measurement of lipid oxidation: a review. J Am Oil Chem Soc 55: 539-544 https://doi.org/10.1007/BF02668066
  5. Yamamoto Y. 1994. Chemiluminescence-based high-performance liquid chromatography assay of lipid hydroperoxides. Methods Enzymol 233: 319-324 https://doi.org/10.1016/S0076-6879(94)33036-0
  6. Johnson JE, Walford R, Harma D, Miquel, J. 1986. Free radicals, aging and degenerative disease. Alen R. Liss, New York, p 99
  7. 최동성, 고가영(역). 2000. 식품기능화학. 지구출판사, 서울
  8. Petra R, Irmgard E, Sabine R, Ibrahim E. 2000. Long-term oral $\beta$-carotene supplementation in patients with cystic fibrosis-Effects on antioxidative status and pulmonary function. Annals of Nutrition & Metabolism 44: 30-37 https://doi.org/10.1159/000012818
  9. Shin MO, Bae SJ, Kim NH. 1992. Antioxidant effect of soyasaponin on the liposomal phospholipid membrane. J Korean Soc Food Nutr 21: 381-385
  10. The Korean Nutrition Society. 2000. Recommended Dietary Allowances for Koreans. 7th revision. p 298
  11. 허 준. 1981. 동의보감. 남산당, 서울
  12. 이시진. 1978. 본초강목. 고문사, 서울. p 412
  13. Yoo HS. 1987. Comparisons of chemical characteristics among P. ginseng, P. grandoflorum and C. lanceolata. MS Thesis. Sookmyung Women's University
  14. Tada T, Kaneiwa Y, Shoji J, Shibat S. 1975. Saponins of the root of P. grandiflorum. Isolation and the stucture of platycodin D. Chem Pharm Bull 23: 2965-2969 https://doi.org/10.1248/cpb.23.2965
  15. Lee EB. 1974. Pharmacological studies on 'Platycodin Radix'. Kor J Pharmacog 5: 49-60
  16. Kubo M, Nagao T, Matsuda H, Namba K. 1986. Immune pharmacological studies on platycodi radix I : Effect on the phagocytosis in the mouse. Shoyagaku Zasshi 40: 367
  17. Sung NY, Lee SJ, Shin JH, Lee IS, Chung YC. 1996. Effects of Platycodon grandiflorum extract on blood glucose and lipid composition in alloxan induced hyperglycemic rats. J Korean Soc Food Sci Nutr 25: 986-992
  18. Kim YS, Lee BE, Kim KJ, Lee YT, Cho KB, Chung YC. 1998. Antitumor and immunodulatory activities of the P. grandiflorum cultivated for more than 20 years. Yakhak Hoeji 42: 382-387
  19. Kim KS, Ezaki O, Ikemoto S, Itakura H. 1995. Effects of Platycodon grandiflorum feeding on serum and liver lipid concentations in rats with diet-induced hyperlipidemia. J Nur Sci Vitamino 41: 485-491 https://doi.org/10.3177/jnsv.41.485
  20. Gregory G. 1984. Liposome technology. CRC Press, Florida. Vol 1, p 139

Cited by

  1. Inhibitory Effect of Extracts of Platycodon grandiflorum (the Ballon Flower) on Oxidation and Nitric Oxide Production vol.18, pp.1, 2011, https://doi.org/10.11002/kjfp.2011.18.1.065
  2. Total Phenolics of Dried Platycodon grandiflorum and Its Effect on Growth of Human Cancer Cell Lines vol.45, pp.1, 2013, https://doi.org/10.9721/KJFST.2013.45.1.84