Cytotoxic and Apoptotic Effects of Soybean and Brown Rice Extracts on Hormone Dependent/lndependent Breast Cancer Cell Lines

대두와 현미 추출몰이 호르몬 의존형 및 비의큰형 유방암세포의 성장에 미치는 영향

  • 성미경 (숙명여자대학교 식품영양학과) ;
  • 박미영 (숙명여자대학교 식품영양학과)
  • Published : 2002.06.01


A number of experimental and epidemiological studies have implicated that antiestrogenic effects of estrogen-like compounds in legumes and plant seeds are responsible for lowering breast cancer risk in human. However, few studies have been conducted to illustrate the possible chemopreventive effects of Korean traditional food materials. This study was performed to determine the cytotoxic and apoptotic effects of yellow soybeans, black soybeans and brown rice extracts on hormone-dependent and hormone-independent human breast cancer cells. Methanol-or acetone-soluble fractions of soybeans or brown rice were incubated with hormone-dependent cells (MCF-7) or hormone-independent cells (MDA-MB-231). Cell cytotoxicity was measured by MTT assay at 24, 48 and 72 hrs of incubation. Apoptotic effects of these extracts toward breast cancer cells were also determined at 48 hrs of incubation by measuring DNA fragmentation. Results indicated that the acetone-soluble fraction of brown rice exerted strongest cytotoxic effect on MCF-7 ceIls, although other fractions also reduced the number of viable MCF-7 cells after 48 hrs of incubation. Both acetone and methanol soluble fractions of all samples exerted a significant cytotoxicity towards MDA-MB-231 cells after 24 hrs of incubation, and acetone and methanol soluble fractions of brown rice were especially effective in these cells. At 48 hrs of incubation, methanol fractions of all three samples induced apopotosis of MDA-MB-231 cells. These results indicate methaol or acetone soluble fractions of yellow soybeans, black soybeans and brown rice induce cytotoxicity in both hormone-dependent and hormone-independent breast cancer cells. Therefore, possible mechanisms of cell cytotoxicity do not necessarily include antiestrogenic effects of soybean or brown rice extract. A possible anticarcinogenic effect of brown rice methanol-soluble fraction may mediated through their apoptotic effect. Further studies are requried to elucidate responsible compounds and mechanisms involved in observed anticarcinogenesis.


  1. The National Cancer Center. 2000. Annual Report of Korean Central Cancer Registry Project
  2. Doll R, Peto R. 1981. The causes of cancer: quantitative estimate of avoidable risks of cancer in the United States today. J Natl Cancer Inst 66: 1191-1308
  3. Miyazak T, Nishijima M. 1981. Structual examination of a water soluble antitumor polysaccharide of Ganoderma Iucidum. Chem Pharm Bull 29: 3611-3616
  4. Lee HP, Gourley L, Duffy SW, Esteve J, Lee J, Day NE. 1991. Dietary effects on breast-cancer risk in Singapore. Lancet 337(8751): 1197-1200
  5. Wu AH, Ziegler RG, Nomura AM, West DW, Kolonel LN, Horn-Ross PL, Hoover RN, Pike MC. 1998. Soy intake and risk of breast cancer in Asians and Asian Americans. Am J Clin Nutr 68: 1437S-1443S
  6. Constantinou AI, Krygier AE, Mehta RR. 1998. Genistein induces maturation of cultured human breast cancer cells and prevents tumor growth in nude mice. Am J Clin Nutr 68: 1426S-1430S
  7. Dixon-Shanies D, Shaikh N. 1999. Growth inhibition of human breast cancer cells by herbs and phytoestrogens. Oncol Rep 6: 1383-1387
  8. Tou JC, Thompson LU. 1999. Exposure to flaxseed or its lignan component during different developmental stages in-fluences rat mammary gland structures. Carcinogenesis 20: 1831-1835
  9. Adlercreutz H, Mousavi Y, Clark K, Hockerstedt K, Wahala K, Makela T, Hase T. 1992. Dietary phytoestrogens and cancer: in vitro and in vivo studies. J Steroid Biochem Mol Biol 42: 331-337
  10. Huang JM, Nasr M, Kim YH, Matthews HR. 1992. Genistein inhibits protein histidine kinase. J Biol Chem 267: 15511- 15515
  11. Adlercreutz H. 1998. Lignans and phytoestrogens. Possible role in cancer. In Progress in Diet and Nutrition. Rozen P, Horwitz C, eds. S. Karger Publishing, Farmington. Vol 14, p 165-176
  12. Vincent A, Fitzpatrick LA. 2000. Soy isoflavones: are they useful in menopause? Mayo Clin Proc 75: 1174-1184
  13. Li Y, Li M, Xing G, Hu Z, Wang Q, Dong C, Wei H, Fan G, Chen J, Yang X, Zhao S, Chen H, Guan K, Wu C, Zhang C, He F. 2000. Stimulation of the mitogen-activated protein kinase cascade and tyrosine phosphorylation of the epidermal growth factor receptor by hepatopoietin. J Biol Chem 275: 37443-37447
  14. Malik RK, Parsons JT. 1996. Integrin-dependent activation of the p70 ribosomal S6 kinase signaling pathway. J Biol Chem 271: 29785-29791
  15. Souci SW, Fachmann W, Kraut K. 2000. Food Composition and Nutrition Tables. CRC press, Boca Raton
  16. Awad AB, Fink CS. 2000. Phytosterols as anticancer dietary components: evidence and mechanism of action. J Nutr 130: 2127-2130
  17. Awad AB, Downie AC, Fink CS. 2000. Inhibition of growth and stimulation of apoptosis by beta-sitosterol treatment of MDA-MB-231 human breast cancer cells in culture. Int J Mol Med 5: 541-545
  18. von Holtz RL, Fink CS, Awad AB. 1998. Beta-sitosterol activates the sphingomyelin cycle and induces apoptosis in LNCaP human prostate cancer cells. Nutr Cancer 32: 8-12
  19. Strom SS, Yamamura Y, Duphorne CM, Spitz MR, Babaian RJ, Pillow PC, Hursting SD. 1999. Phytoestrogen intake and prostate cancer: a case-control study using a new database. Nutr Cancer 33: 20-25
  20. van Rensburg SJ, Hall JM, Bruyn DB. 1985. Effects of various dietary staples on esophageal carcinogenesis induced in rats by subcutaneously administered N-nitrosomethylbenzylamine. J Natl Cancer Inst 75: 561-566
  21. Hudson EA, Dinh PA, Kokubun T, Simmonds MS, Gescher A. 2000. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiol Biomarkers Prev 9: 1163-1170
  22. Li Y, Bhuiyan M, Sarkar FH. 1999. Induction of apoptosis and inhibition of c-erbB-2 in MDA-MB-435 cells by genistein. Int J Oncol 15: 525-533
  23. Balabhadrapathruni S, Thomas TJ, Yurkow EJ, Amenta PS, Thomas T. 2000. Effects of genistein and structurally related phytoestrogens on cell cycle kinetics and apoptosis in MDA-MB-468 human breast cancer cells. Oncol Rep 7: 3-12

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