Study of Macrophage Stimulating Activity of the Polysaccharide Isolated from Leaves of Carthamus tinctorius L.

홍화(Carthamus tinctorius L.)잎으로부터 분리한 다당류의 Macrophage 활성화에 대한 연구

  • Kwak, Ji-Eun (Graduate School of Natural Resources, Korea University) ;
  • Kim, Kyung-Im (Graduate Shool of Biotechnology, korea University) ;
  • Jeon, Hyuck (Graduate Shool of Biotechnology, korea University) ;
  • Hong, Bum-Shick (Graduate Shool of Biotechnology, korea University) ;
  • Cho, Hong-Yon (Graduate Shool of Biotechnology, korea University) ;
  • Yang, Han-Chul (Graduate Shool of Biotechnology, korea University)
  • 곽지은 (고려대학교 자연자원대학원) ;
  • 김경임 (고려대학교 생명공학원) ;
  • 전혁 (고려대학교 생명공학원) ;
  • 홍범식 (고려대학교 생명공학원) ;
  • 조홍연 (고려대학교 생명공학원) ;
  • 양한철 (고려대학교 생명공학원)
  • Published : 2002.06.01


In the screening of Korean traditional tea sources for the cellular lysosomal enzyme activity of peritoneal macrophage from mice, CT-0, a cold-water extract from Carthamus tinctorius L., showed the highest macro-phage-stimulating activity. CT-1-IIa-2-1, a purified macrophage-stimulation polysaccharide was obtained by a series of purification steps such as anion exchage chromatography with DEAE-Toyopearl 650M, gel permeation chromatography with Sepharose CL-6B, Sephacryl S-200, and HPLC with Superdex G-75. The molecular weight of homogeneous purified polysaccharide was estimated about 68 kDa. CT-1-IIa-2-1 consisted of xylose 27.44%, arabinose 16.14%, mannose 15.92% and glucose 14.47%. To measure acute toxicity, dose of 50, 100, 500, and 1000 mg/kg were intraperitoneally injected to ICR mice. The LD$\_$50/ was about 397 mg/kg.


  1. Bae JH. 1997. Effect of Ganoderma lucidum on the IL-1, TNF and IL-12 gene expression of macrophages. J Korean Soc Food Sci Nutr 26: 978-982
  2. Kim SW. 1998. Studies on anti-microbial and anti-cancer functions of polysaccharide extracted from Ganoderma lucidum. J Korean Soc Food Sci Nutr 27: 1183-1188
  3. Roitt I, Brostoff J, Male D. 1993. Immunology. 3rd ed. Mosby, New York
  4. Brattgjerd S, Evensen Q, Lauve A. 1994. Effect of injected yeast glucan on the activity of macrophage in Atlantic salmon, Salmo salar L., as evaluated by in vitro hydrogen peroxide production and phagocytic capacity. Immumology 83: 288-294
  5. Kim MN, Kim HH. 1995. Analysis of safflower by high- performance liquid chromatography. J Korean Ind & Eng Chemistry 6: 238-242
  6. Dubois M, Gilles KA, Hamilton JK, Reber PA, Smith F. 1956. Colorimeter method for determination of sugar and related substances. Anal Chem 28: 350-356
  7. Blumemkrantz N, Asboe-Hansen G. 1973. New method for quantitative determination of uronic acids. Anal Biochem 54: 484-489
  8. Lowry OH, Rosebrough NJ, Farr AL, Randoll RJ. 1951. Protein measurement with the folin phenol reageant. J Biol Chem 193: 265-270
  9. Jones TM, Albersheim P. 1972. A gas chromatographic method for the determination of aldose and uronic acid constituents of plant cell wall polysaccharides. Plant Physiol 49: 926-936
  10. Suzuki I, Tanaka H, Kinoshita A, Oikawa S, Osawa M, Yadomae T. 1990. Effect of orally administerd $\beta$-glucan on mactophage function in mice. Int J Immunopharmac 12: 675-684
  11. Keller R, Keist R, Wechsler H, Leist TP, van der Meide PH. 1990. Mechanisms of macrophage-mediated tumor cell killing : a comparative analysis of the roles of reactive nitrogen intermediates and tumor necrosis factor. Int J Cancrt 46: 682-686
  12. Yamada H, Kiyohara H, Cyong JC, Takemoto N, Komatsu Y, Kawamura H, Aburada M, Hosoya E. 1993. Fractionation and characterization of mitogenic and anti-complementary active fraction from Kampo (Japanese herbal). Kor J Pharmacog 13: 62-69
  13. Chang IM, Kim JH, Han DS. 1983. Toxicological evaluaion of medicianl plants used for herbal drugs (Ⅳ) Acute toxicity and antitumor activities. Kor J Pharmacog 13: 62-69
  14. KFDA. 1983. 독성시험 표준작업지침서. p 218-246
  15. Franz G. 1989. Polysaccharides in pharmacy, current applications and future conceps. Planta Med 55: 493-497
  16. Kiyohara H, Yamada H. 1994. Characterisation of methyl- ester distributions in galacturonan regions of complement activation pectins from the roots of Angelica acutiloba Kitagawa. Carbohydrate Polymers 25: 117-122
  17. Yamada H, Sun XB, Matsumoto T, Ra KS, Hirano M. 1991. Purificarion of anti-urcer polysaccharides from the roots of Bupleurum falcatum. Planta Med 57: 554-559
  18. Kim SW. 1993. Effect of polysaccharide extracted from Ganderma lucidum on immunological activities of macrophage. PhD Thesis. Dankuck University of Korea
  19. Nathan CF, Silverstein SC, Brukner LH, Cohn ZA. 1979. Extracellular cytolysis by activated macrophages and granulocytes. J Exp Med 149: 100-113
  20. Kim KI, Kim JW, Hong BS, Shin DH, Cho HK, Yang HC. 2000. Antitumor, genotoxicity and anticlastogenic antivities of polysaccharide from Curcuma zedoaria. Mol Cells 10: 392-398