Effects of Bacillus polyfermenticus SCD Administration on Fecal Microflora and Putrefactive Metabolites in Healthy Adults

  • Published : 2002.08.01


Probiotics have been suggested to improve gastrointestinal health in humans. To investigate the effects of Bacillus polyfermenticus SCD administration on fecal microflora and putrefactive metabolites in humans, Bacillus polyfermenticus SCD (4.00${\times}$10$\sub$5/ CFU/mg) was administrated to ten healthy subjects (5 men and 5 women, average age 24 years) three times a day for 2 weeks. Fecal samples were collected before (1st and 2nd weeks, control), during (3rd and 4th weeks), and 2 weeks after the administration. The fo11owing microbial groups were evaluated in the feces: aerobic and anaerobic bacteria, Bacillus polyfermenticus SCD, Lactobacillus, Bifidobacterium, total lactic acid bacteria, Salmonella, Clostridium, Clostridium perfringens, Eubacterium, Staphylococcus, Coliform bacteria, Pseudomunas, and Yeast. Fecal concentrations of total aerobic bacteria (p<0.05, p<0.01, 3rd and 4th weeks), total lactic acid bacteria (p<0.01, 3rd, 4th and 5th weeks), and Bifidobacteria (p<0.05, 4th and 5th weeks) were significantly increased in all subjects, compared to the control, from the 3rd week after the administration of the products. Clostridium (p<0.01, 4th week), Clostridium perfringens (p<0.05, p<0.01, 3rd and 4th weeks), and coliform (p<0.01,5th week) were significantly reduced from the 3rd week of administration. No significant changes in the fecal concentrations of Pseudomonas, Lactobacillus, Eubacterium, Staphylococcus, yeast, and total anaerobes were observed. Six weeks after the administration, the concentration of all rnicroorganlsrns returned to the basal level. Bacillus polyfermenticus SCD was significantly maintained from the 3rd week to 6th week of the study. Despite the absence of a statistical significance, the putrefactive metabolites (ammonia, indole, skatole, and $\rho$-cresol) and the pH value tended to be lower during and after the test periods than the base line. These results show that this probiotic preparation is able to colonize the intestine, and suggest that it may be useful as a beneficial probiotic in humans.


  1. J. Appl. Microbiol. v.91 Increased resistance of mice to Salmonella enterica serovar typhimurium infection by symbiotic administration of bifidobacteria and transgalactosylated oligosaccharides Asahara, T.;K. Nomoto;K. Shimizu;M. Watanuki;R. Tanaka https://doi.org/10.1046/j.1365-2672.2001.01461.x
  2. Microbiol. Immunol. v.36 Impact of Bifidobacterium longum on human fecal microflora Benno, Y.;T. Mitsuoka https://doi.org/10.1111/j.1348-0421.1992.tb02071.x
  3. Kor. J. Vet. Res. v.40 In vitro selection of lactic acid bacteria for probiotic use in pigs Byun, J. W.;G. T. Kim;H. S. Bae;Y. J. Baek;W. K. Lee
  4. Kor. J. Appl. Microbiol. Biotechnol. v.23 Effects of the water extract of Akebia on the growth of Clostridium perfringens and some intestinal microorganism Han, B. J.;S. K. Woo;H. K. Shin
  5. Kor. J. Environ. Biol. v.15 Fecal microflora of college students in JeonBuk area Jin, H. S.
  6. Kor. J. Nutr. v.32 Relations between the microfloral composition and the environmental factors affecting Korean infants during lactation Jin, H. S.;K. J. Lee;S. J. Moon
  7. Kor. J. Dairy Sci. v.21 Effects of lactic acid bacteria on infection of Salmonella typhimurium in mouse Jun, H. S.;Y. K. Choi;Y. S. Won;B. H. Hun;J. W. Kim
  8. Kor. J. Appl. Microbiol. Biotechnol. v.28 Microbiological identification of medical probiotic Bispan strain Jun, K.-D.;K.-H. Lee;W.-S. Kim;H.-D. Paik
  9. Kor. J. Food Sci. Technol. v.4 Effects of the fructooligosaccharide intake on human fecal microflora and fecal properties Kang, K. H.;K. M. Kim;S. G. Choi
  10. J. Kor. Soc. Food Sci. Nutr. v.29 Carbon dioxide production and quality changes in Korean fermented soybean paste and hot pepper-soybean paste Kim, G. T.;Y. I. Hwang;S. I. Lim;D. S. Lee
  11. Lett. Appl. Microbiol. v.29 Lactococci as probiotic strains: Adhesion to human enterocyte-like Caco-2 cells and tolerance to low pH and bile Kimoto, H.;J. Kurisaki;N. M. Tsuji;S. Ohmomo;T. Okamoto https://doi.org/10.1046/j.1365-2672.1999.00627.x
  12. Lett. Appl. Microbiol. v.32 Partial characterization of polyfermenticin SCD, a newly identified bacteriocin of Bacillus polyfermenticus Lee, K.-H.;K.-D. Jun;W. -S. Kim;H.-D. Paik
  13. Kor. J. Microbiol. Biotechnol. v.27 Effect of Bifidobacterium longum HY8001 administration on human fecal bacterial enzymes and microflora Lee, W. K.;S. M. Lee;H. S. Bae;Y. J. Baek
  14. Immunol. Cell Biol. v.78 Modulating immune responses with probiotic bacteria Matsuzaki, T.;J. Chin https://doi.org/10.1046/j.1440-1711.2000.00887.x
  15. Anaerobe v.5 Antagonism against anaerobic and facultative bacteria through a diffusible inhibitory compound produced by a Lactobacillus sp. isolated from the rat fecal microbiota Nardi, R. D.;A. R. M. Santos;M. A. R. Carvalho;L. M. Farias;L. C. Benchetrit;J. R. Nicoli https://doi.org/10.1006/anae.1999.0217
  16. J. Microbiol. Biotechnol. v.10 Identification and partial characterization of cerein BS 229, a bacteriocin produced by Bacillus cereus BS 229 Paik, H.-D.;N.-K. Lee;K.-H. Lee;Y. -I. Hwang;J.-G. Pan
  17. Anaerobe v.3 Cell-free wheys from bifidobacteria fermented milks exert a regulatory effect on the intestinal microflora of mice and humans Romond, M. B.;A. Yazourh;C. Romond https://doi.org/10.1006/anae.1997.0090
  18. Kor. J. Microbiol. Biotechnol. v.26 Changes of the intestinal microflora and fecal properties by intake of yoghurt added capsulated or uncapsulated Bifidobacteria Ryu, B. H.;S. H. Cho;S. W. Ha;K. M. Park;K. H. Kang
  19. Kor. J. Food Sci. Technol. v.31 Selection and characteristics of Lactobacillus acidophilus isolated from Korean feces Shin, M. S.;H. M. Kim;G. T. Kim;C. S. Huh;H. S. Bae;Y. J. Baek
  20. Kor. J. Appl. Microbiol. Biotechnol. v.24 Effects of the lactic acid bacteria administration on fecal microflora and putrefactive metabolites in healthy adults Shin, M. S.;Y. J. Kim;H. S. Bae;Y. J. Baek
  21. Anaerobe v.7 Effect of Lactobacillus paracasei on intestinal colonisation of lactobacilli, bifidobacteria and Clostridium difficile in elderly persons Sullivan, A.;A. C. Palmgren;C. E. Nord https://doi.org/10.1006/anae.2001.0377
  22. Anal. Biochem. v.236 A gas chromatographic analysis of fecal short-chain fatty acids, using the direct injection method Tangerman, A.;F. M. Nagengast https://doi.org/10.1006/abio.1996.0123
  23. Ailment Pharmacol. Ther. v.13 Impact on the composition of the fecal flora by a new probiotic preparation: Preliminary data on maintenance treatment of patients with ulcerative colitis Venturi, A.;P. Gionchetti;F. Rizzello;R. Johansson;E. Zucconi;P. Brigidi;D. Matteuzzi;M. Campieri https://doi.org/10.1046/j.1365-2036.1999.00560.x
  24. Agric. Biol. Chem. v.41 Gas chromatographic microdetermination of indole and skatole in gastrointestinal contents of domestic animals Yoshihara, I.;K. Maruta https://doi.org/10.1271/bbb1961.41.2083
  25. Agric. Biol. Chem. v.42 Gas chromatographic detection of volatile phenols and microdetermination of ρ-cresol in gastrointestinal contents of domestic animals Yoshihara, I. https://doi.org/10.1271/bbb1961.42.1607