Optimization for Hot Water Extraction Process of Cordyceps militaris using Response Surface Methodology

반응표면분석법에 의한 동충하초 열수추출공정의 최적화

  • Published : 2002.06.01

Abstract

This study was conducted to optimize hot water extraction process of cordyceps militaris for development beverage. Optimal condition for hot water extraction was investigated with changes in extraction concentration and temperature by response surface methodology. The content of soluble solid was effected with concentration. The efficiency of extraction for turbidity, total sugar, reducing sugar and protein increased with low concentration. The response variables had significant with concentration and the established polynomial model was suitable (p>0.05) model by lack-of-fit analysis. Optimal extraction conditions as the limited renditions of 2.0∼2.4% extractable solids, 1.5∼2.0% brix, l14∼120 $\mu\textrm{g}$/g total sugar, 10.5∼11.0 $\mu\textrm{g}$/g reducing sugar and 110∼l15 $\mu\textrm{g}$/g protein were 95∼100$\^{C}$ and 4.0∼4.1% of concentration.

References

  1. Kang, K.C., Park, J.H. Baek, S.B., Jhin, H.S. and Rhee, K.S. (1992) Optimization of bebrage preparation from Schizandra chinensis baillon by response surface methodology, Korean J. Food Sci, Technol., 24, 74-81
  2. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956) Colorimetric method for determination of sugar and related substance. Anal. Chem., 28, 350-352 https://doi.org/10.1021/ac60111a017
  3. Lee, G.D., Lee, J.E. and Kwon, J.H. (2000) Application of response surface methodology in food chemistry, Food and Industry, 33, 33-45
  4. Kim, S.D., Ku, Y.S, Lee, I.Z, Park, I.K. and Youn K.S. (2001) Optimization for hot water extraction condition of Liriope spicata tuber using response surface methodology, Korean J. Postharvest Sci. Technol., 8, 157-163
  5. Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-428 https://doi.org/10.1021/ac60147a030
  6. Han D.S., Song, A.N. and Kim Y.E. (1999) State, problem and research direction of Cordyceps sp. Food Science and Industry, 32, 67-82
  7. Lowry, O.H. and Rosebrough, N.J. (1951) Protein measurement with the folin-phenol reagents. J. Biol. Chem., 193, 256-265
  8. Shim, J.Y., Lee Y.S., Lim, S.S. Shin, K.H. Hyun, J.E., Kim, S.Y. and Lee, E.B. (2000) Pharmacological activities of Paecilomyces japonica, a new type Cordyseps sp. Kor. J. Pharmacogn., 31, 163-168
  9. 한대석, 송효남, 김상희 (1998) 동충하초 새로운 기능성 식품소재, 식품기술 11, 107-116