Neuroprotecticve Effect of Rhei Rhizoma on Transient Global Ischemia in Gerbil

Gerbil의 전뇌허혈에 대한 대황의 신경보호효과

  • Bum-Hoi, Kim (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Hyuk-Sang, Jung (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Ran, Won (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Ji-Ho, Park (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Chul-Hun, Kang (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Nak-Won, Sohn (Department of Neuroscience, Graduate School of East-West Medical Science, Kyung Hee University)
  • Published : 2002.09.01

Abstract

목적 : 본 실험에서는 gerbil을 이용한 전뇌허혈 동물모델에서 뇌허혈손상 직후 지연성 뇌손상에 대한 대황의 방어효과와 Apoptosis 과정중의 Bax와 Bcl-2 단백질에 대한 조절작용을 관찰하고, TUNEL 염색법을 통하여 대황이 gerbil hippocampus CAl영역의 pyramidal neuron의 세포사에 미치는 영향과 PCl2세포를 이용한 세포배양 모델에서의 대황의 신경방어 효과를 관찰하였다. 방법 : Mongolian gerbil의 총경동맥을 5분간 폐색하여 가역성 전뇌허혈을 유발시킨 후 대황의 전탕액을 하루에 한번 경구 투여하였다. 대황의 신경 보호 효과는 수술 7일 후에 cresyl violet으로 염색하여, 살아있는 신경 세포의 수를 세어 측정하였다. 또, 수술 3일 후에는 면역조직화학적 방범을 통하여 Bax. Bcl-2단백질의 발현과 대황의 신경보호 효과와의 관련성을 알아보았다. 결과: 가역적 전뇌허혈이 일어난 동물군의 경우 hippocampus의 CAl 영역에서 살아있는 신경세포의 수는 $51.0{\pm}2.5개{\;}/mm$에 불과하였으나, 그에 비해 수술 후 7일간 대황을 투여한 동물군은 $106.2{\pm}2.5개{\;}/mm$로 살아 있는 신경세포수가 크게 증가하였다. Apoptosis를 촉진하는 단백질인 Bax의 발현은 3일간 대황을 투여한 동물군의 경우 hippocampus의 CAl 영역에서 현저하게 저해되었고, Apoptosis를 억제하는 Bcl-2 단백질의 발현은 변화가 없었다. TUNEL assay를 통하여 살펴본 결과 대황 투여군의 apoptotic 신경세포사가 감소하였으며 이는 Bax protein의 발현과 유사한 양상을 나타내었다. 결론:대황이 Bax 단백질의 발현을 억제하여 상대적으로 Bax/Bcl-2 자연적 세포사를 억제하여 Mogolian gerbil의 가역성 전뇌허혈 모델에서 신경보호효과를 나타내는 것으로 사료된다.

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