Analysis of Cyclofenil and its Metabolites in Human Urine using GC/MS

GC/MS를 이용한 소변중의 Cyclofenil 및 그 대사체의 분석

  • Myung, Seung-Woon (Donping Control Center, Korea Institute of Science and Technology) ;
  • Min, Hye-Ki (Donping Control Center, Korea Institute of Science and Technology) ;
  • Cho, Hyun-Woo (Department of Chemistry, KyongGi University) ;
  • Kim, Dong-Hyun (Donping Control Center, Korea Institute of Science and Technology) ;
  • Kim, Myungsoo (Donping Control Center, Korea Institute of Science and Technology)
  • 명승운 (한국과학기술연구원 도핑콘트롤센터) ;
  • 민혜기 (한국과학기술연구원 도핑콘트롤센터) ;
  • 조현우 (경기대학교 이과대학 화학과) ;
  • 김동현 (한국과학기술연구원 도핑콘트롤센터) ;
  • 김명수 (한국과학기술연구원 도핑콘트롤센터)
  • Received : 2002.01.09
  • Published : 2002.06.25

Abstract

International Olympic Committee (IOC) prohibits the use of cyclofenil for male. To prove whether cyclofenil product was taken or not, the analytical method in urine using GC/MS was established. The extraction recoveries of cyclofenil and its metabolites were higher in the range of pH 5-9. Because the parent drug (cyclofenil) was not excreted in urine, metabolites were detected and theses were used as a criteria of cyclofenil dose. Therefore the metabolites were hydrolyzed, extracted at pH 9.6, derivatized with MSTFA and analyzed by GC/MS.

Keywords

cyclofenil;metabolite;doping;GC/MS

Acknowledgement

Supported by : 과학기술부

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