Analysis of Endotoxin Using Analytical Conditions of Optical Density in Metalworking Fluid Sample

금속가공유 시료에서 일부 Optical Density 설정값에 따른 엔도톡신의 정량

  • Received : 2002.04.26
  • Published : 2002.10.25

Abstract

This study was performed to identify the proper analytical conditions of endotoxins regarding optical density in the workplace where metalworking fluids are used. This study found that "onset time method" was more accurate than "time to $V_{max}$ method". Reproducibility and accuracy analyzed by "onset time method" was greatly higher than the "time to $V_{max}$". The optical density of "0.03" was the most appropriate analytical condition among "onset time method". In this analytical condition, linearity of 0.998 was obtained and recovery rate ranged from 88% to 105% at the endotoxin concentrations below 5 EU/mL. No significant difference of endotoxins was observed between the optical densities of "0.03" and "0.05". However, correlation coefficients were different with statistical significance (p<0.01). This study confirmed that either the optical densities "0.03" or "0.05" should be used to analyze endotoxin. Of these optical density values, OD with correlation coefficient higher than 0.98 should be used to analyze endotoxin in environmental samples.

Keywords

Metalworking fluids;endotoxin;optical density;Onset time method;time to $V_{max}$

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Acknowledgement

Supported by : 한국방송통신대학교