Study on Metabolism and Excretion of Carteolol in Human Urine using GC/MS

GC/MS를 이용한 carteolol의 대사 및 배설연구

  • Received : 2002.08.22
  • Published : 2002.10.25

Abstract

International Olympic Committee (IOC) prohibits the use of carteolol which is one of ${\beta}$-blockers. To prove whether carteolol product was taken or not, the analytical method in urine using GC/MS was established, and metabolism and excretion study were evaluated. As compared with acid hydrolysis, enzyme hydrolysis method was more efficiency. Coefficients of variation for intra-assay precision was around 10%. Error was less than 5% except the concentration of $0.05{\mu}g/m{\ell}$. Recovery was 78.5% at $2{\mu}g/m{\ell}$. Free carteolol, conjugated carteolol, and small amount of p-OH carteolol were found in dosed human urine samples. The conjugated form was being 59.4% of the total carteolol in human urine. The amount of carteolol renal excreted for 72 h after oral dose of 10 mg of carteolol was 49% of the administred dose.

Keywords

carteolol;metabolite;doping;GC/MS

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Acknowledgement

Supported by : 과학기술부