Characteristics of β-casein Gene using the PCR Technique in Korean Native Goat

PCR 기법을 이용한 한국재래산양 β-casein 유전자의 특성

  • Kim, Ji-Ae (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Ryoo, Seung-Heui (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, Sung-Lan (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, Jun-Heon (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Gil-Woong (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Sun-Kyun (Department of Animal Science, Woosong Information College) ;
  • Sang, Byung-Chan (Division of Animal Science and Resources, College of Agriculture and Life Sciences, Chungnam National University)
  • 김지애 (충남대학교 농업생명과학대학 동물자원학부) ;
  • 류승희 (충남대학교 농업생명과학대학 동물자원학부) ;
  • 유성란 (충남대학교 농업생명과학대학 동물자원학부) ;
  • 이준헌 (충남대학교 농업생명과학대학 동물자원학부) ;
  • 서길웅 (충남대학교 농업생명과학대학 동물자원학부) ;
  • 김선균 (우송정보대학 동물과학과) ;
  • 상병찬 (충남대학교 농업생명과학대학 동물자원학부)
  • Published : 2002.12.31

Abstract

This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from analysis of genetic characteristics of $\beta$-casein gene in Korean Native goat and Saanen using the PCR-RFLP. This study confirmed the amplified products of 481bp fragments obtained from the amplification of $\beta$-casein loci by PCR. The $\beta$-casein AB genotype showed 481, 284 and 197bp, and $\beta$-casein BB genotype showed 284 and 197bp fragments in Korean Native goat and Saanen. The frequencies of $\beta$-casein genotype in Korean Native goat were 6.25 and 93.75% for AA and AB and the frequencies of $\beta$-casein genotype in Saanen were 57.14 and 42.86% for AA and AB types. The frequencies of $\beta$-casein A and B alleles were 0.031 and 0.969 in Korean Native goat and the frequencies of $\beta$-casein A and B alleles are 0.286 and 0.714 in Saanen, respectively. The nucleotide sequence of $\beta$-casein gene of Korean Native goat was 97.71% higher homology with 11 nucleotide sequences difference of that of goat reported in GeneBank (M90556). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphism and sequencing for $\beta$-casein gene should be used as basic and applying data for preservation and improvement of genetic resources in Korean Native goat breeding.