Antibacterial Activity of Sea-mustard, Laminaria japonica Extracts on the Cariogenic Bacteria, Streptococcus mutans

충치균 (Streptococcus mutans)에 대한 다시마 추출물의 항균활성

  • Kim Ji Hoe (National Fisheries Research & Development Institute) ;
  • Lee Doo Seog (National Fisheries Research & Development Institute) ;
  • Lim Chi Won (National Fisheries Research & Development Institute) ;
  • Park Hee Yeon (National Fisheries Research & Development Institute) ;
  • Park Jeong Heum (National Fisheries Research & Development Institute)
  • Published : 2002.03.01


In previous reports the authors have screened the inhibition effects of marine algae extracts on halitosis, and demonstrated that a brown algae, Eisenia bicyclis (' Daehwang') possess not only strong deodorant effect bug also considerable anticariogenic activities. In this study, we screened antibacterial effects of various marine algae, and measured minimum inhibitory concentration (MIC) value of them against mutans streptococci in vitro. Among the 27 species of marine algae, $80\%$ ethanol extract of dried sea-mustard, Laminaria japonica ('Dasima') showed the strongest inhibition activity against Streptococcus mutans KCTC 3300. The extracts of Ulva lactuca ('Galparae'), Codium fragile ('Cheonggak'), Ecklonia cava ('Gamtae'), E. stolonifera ('Gompi') and Undalia Pinnatifida ('Miyeok') showed slightly weaker inhibitory potency than L. japonica. Differences of MIC values in $80\%$ ethanol extract of some species of marine algae were observed depending on test bacterial species, i.e., S. mutans KCTC 3300 or S. sobrinus KCTC 3307. Eighty percent ethanol extract of dried L japonica was fractionated with diethyl ether, chloroform, ethyl acetate, n-buthanol and water successively, The ether-soluble fraction had inhibitory effect on S. mutans KCTC 3300, however the inhibitory effects were not found in the other fractions. The MIC values of $80\%$ ethanol extract and ether fraction were 180 and 105 $\mu$g/mL respectively, while no significant inhibition activity of water-soluble fraction was found even when the fraction was added up to 5,500 $\mu$g/mL.

우리 나라 연안에서 채취한 해조류 27종의 $80\%$ ethanol 추출물이 Streptococcus mutans KCTC 3300의 증식에 미치는 영향을 검색하고, 다시마의 추출용매별 항균성과, $80\%$ ethanol 추출물을 용매분획하였을 때 획분별 MIC를 측정하였다. S. mutans KCTC 3300에 대한 해조류 추출물의 항균활성은 다시마가 가장 강하였고, 갈파래, 청각, 감태, 곰피 및 미역도 비교적 높은 활성을 나타내었으며 홍조류보다 갈조류와 녹조류에 속하는 종이 대체적으로 강하였다 다시마의 항균활성은 추출용매에 따라 차이가 있었으나 용매별 가용성 획분의 량과 항균성간에는 뚜렷한 관련이 나타나지 않았다. 5. mutans KCTC 3300과 S. sobrinus KCTC 3307에 대한 해조류의 $80\%$ ethanol 추출물의 MIC는 해조류의 종뿐만 아니라 동일한 해조류 추출물의 경우에도 균종에 따라 차이가 있었다. 다시마의 $80\%$ ethanol 추출물을 유기용매를 사용하여 순차 분획하였을 때 항균활성 물질의 대부분은 ether 획 분에 이행하여 이 획분의 MIC는 105$\mu$g/mL이었고, 수용성 획분은 약 5,500$\mu$g/mL의 농도에서도 균의 증식을 저지하지 못하였다.



  1. Baik, S.E. and J.W. Kang. 1986. Antimicrobial activity of the volatile and lipid fractions of marine algae. Korean J. Phycol., 1, 293-310 (in Korean)
  2. Cho, S.Y., B.J. You, M.H. Chang, S.J. Lee, N.J. Sung and E.H. Lee. 1994. Screening for antimicrobial compounds in unused marine resources by the paper disk method. Korean J. Food Sci. Technol., 26, 261-265 (in Korean)
  3. Endo, A., O. Hayashida and S. Murakawa. 1982. Mutasein, a new inhibitor of adhesive-insoluble glucan synthesis by glucosyItransferase of Streptococcus mutans. J. Antibiot., 36, 203-207
  4. Hardie, J.M. and R.A. Whiley. 1992. The genus Stneptococcus-Oral. In The Prokaryotes, 2nd ed. A. Balows, H.G. Truper, M. Dworkin, W. Harder and K.H. Schleifer, ed. Springer-Verlag, New York, pp. 1421-1449
  5. Harty, D.W.S., H.J. Oakey, M. Patnkakis, E.B.H. Hume and K.W. Knox. 1994. Pathogenic potential of lactobacilli. Int. J. Food Microbiol., 24, 179-189
  6. Kim, S.H., S.B. Lim, Y.H. Ko, C.K. Oh, M.C. Oh and C.S. Park. 1994. Extraction yields of Hizikia fusiforme by solvents and their antimicrobial effects. Bull. Korean Fish. Soc., 27, 462-468 (in Korean)
  7. Kurihara, H., Y. Goto, M. Aida, M. Hosokawa and K. Takahashi. 1999. Antibacterial activity against cariogenic bacteria and inhibition of insoluble glucan production by free fatty acids obtained from dried Gloiopeltis furcata. fisheries Science, 65, 129-132
  8. Kwon, I.B., Y.W. Lee, B.J. An and S.Y. Lee. 1993. Inhibitory effect of cacao bean husk extract on glucosyltransferase from Streptococcus mutans B13. Korean J. Biotechnol. Bioeng, 8, 75-82 (in Korean)
  9. Lee, D.S., S.B. Kim, T.J. Kim, J.H. Kim, C.I. Ji and J.H. Park. 1999. Deodorant effect of marine algae extracts on halitosis. Bull. Nat'l. Fish. Res. Dev. Inst. Korea, 57, 195-201 (in Korean)
  10. Lee, D.S., T.J. Kim, J.H. Kim, S.B. Kim, S.W. Cho, C.W. Lim and J.G. Min. 2001. Effect of Eisenia bicyclis extract on the growth and glucosyltransferase activity of Streptococcus mutans. Bull. Nat'l Fish. Res. Dev. Inst. Korea, 59, 171-176 (in Korean)
  11. Lee, K.Y., H.S. Cho, J.W. Yoon and T.R. Hae. 1993. Study on the development of preventive agent of dental canes from biological active materials. I. Development of disc PAHA for an artificial tooth and preventive effect on dental caries from plant extracts. Korean J. Biotechnol. Bioeng., 8, 126-132 (in Korean)
  12. Lim, C.W., J.S. Lee, K.T. Son, J.H. Park and Y.J. Cho. 1999. Antimicrobial properties of extracts of the red alga, Symphyocladia latiuscula. Bull. Nat'l. Fish. Res. Dev. Inst. Korea, 57, 211-220(in Korean)
  13. Lorian, V. 1991. Antibiotics Laboratory Medicine. Williams & Wilkins, Baltimore, pp. 17-105
  14. Nakahara, K., S. Kawabata, H. Ono, K. Ogura, T. Tanaka, T. Ooshima and S. Hamada. 1993. Inhibitory effect of Oolong tea polyphenols on glucosyltransferases of mutans streptococci. Appl. Environ. Microbiol., 59, 968-973
  15. Nisizawa, K. 1978. Marine algae from a viewpoint of pharmaceutical studies. Jap. J. Phycol., 26, 73-78
  16. Oh, C.K., M.C. Oh, S.H. Kim, S.B. Lim and S.H. Kim. 1998. Antimutagenic and antimicrobial effect of ethanol extracts from seamustard and sea-tangle. J. Korean Fish. Soc., 31, 90-94 (in Korean)
  17. Sakanaka, S., M. Kim, M. Taniguchi and T. Yamamoto. 1989. Antibacterial substances bacterium. Agric. Biol. Chem, 53, 2307-2311
  18. Uchida, A., A. Shimada and Y. Ishida. 1988. Antibactenal and antialgal substances produced by the dinoflagellate Peridinium bipes. Nippon Suisan Gakkaishi, 54, 1941 - 1945
  19. You, Y.S., K.M. Park and Y.B. Kim. 1993. Antimicrobial activity of some medical herbs and spices against Streptococcus mutans. Kor. J. Appl. Microbiol. Biotechnol., 21, 187-191 (in Korean)
  20. 김대범, 주훈, 백병주, 송완엽, 송요한. 1995. Streptococcus mutans의 우식 활성에 미치는 propolis의 영향. 대한소아치과학회지, 22, 231-238
  21. 김옥선, 주동식, 어명회, 이정석, 조순영. 2001. 강릉지역 토종 다시마 추출물의 항균활성. 2001 년도 추계 수산관련학회 공동학술대회 발표요지집, pp. 181-182
  22. 佐藤 實. 1993. 海藻成分の機能性. In 海藻の科學, 大石圭一 編. 朝倉書店, pp. 160-181

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