Immunohistochemical Localization of Nerve Growth Factor, Glial Fibrillary Acidic Protein and Ciliary Neurotrophic Factor in the Forebrain of the Developing Mongolian Gerbil

발생중인 Mongolian gerbil의 전뇌에서 NGF, GFAP 및 CNTF의 분포

  • Park, Il-Kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kyoug-Youl (College of Veterinary Medicine, Chungnam National University) ;
  • Song, Chi-Won (College of Veterinary Medicine, Chungnam National University) ;
  • Kwon, Hyo-Jung (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Mi-Sun (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Mi-Young (College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Young-Gil (College of Medicine, Konyang University) ;
  • Lee, Chul-Ho (Korean Research Institute of bioscience and biotechnology (KRIBB)) ;
  • Ha, Kwon-Soo (College of Medicine, Kangwon National University) ;
  • Lee, Kang-Yi (College of Oriental Medicine, Daejeon University) ;
  • Kim, Moo-Kang (College of Veterinary Medicine, Chungnam National University)
  • Accepted : 2002.06.10
  • Published : 2002.06.29


The immunohistochemical localization of the nerve growth factor (NGF), glial fibrillary acidic protein (GFAP) and ciliary neurotrophic factor (CNIF) in the developing Mongolian gerbil forebrain was investigated by the immunohistochemical and electron microscopy methods. Generally, the NGF specifically recognizes the neurons, the GFAP does the glia, and the CNIF does the motor neurons. This study demonstrates the location of the NGF, GFAP and CNTF in the developing Mongolian gerbil from the embryonic days 17 (E17) to the postnatal weeks 3 (PNW 3). The NGF was localized at E19 in the olfactocy bulb and the cerebral cortex, expanded to the hippocampus, and the diagonal bond from the late prenatal period to PNW 3. GFAP was observed in the lateral ventricle and the third ventricle at E17, projected into the cerebral cortex at E19. The GFAP was observed to have the largest numbers in several parts of the forebrain at the postnatal days 2 (PND2), while the most numerous CNTF was observed at PNW 2. The CNTF-IR cells were observed only in the postnatal days and were found in the olfactory bulb, cerebral cortex both neuron and neuroglia at PND3. Electron microscopy showed that the NGF, GFAP and CNTF were not related to any connections with any particular subcellular structure. These results suggest that NGF, GFAP and CNTF be related to the neuron and neuroglia at the prenatal and postnatal stages in the developing Mongolian gerbil.


Supported by : 한국과학재단


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