Matrix solid phase dispersion isolation and high performance liquid chromatographic determination of five benzimidazole anthelmintics in bovine muscle, liver and omasum

시료고체상분산처리와 액체크로마토그라피를 이용한 소의 근육, 간 및 천엽에서의 벤지미다졸계 구충제 잔류분석

  • Kim, Chung-Hui (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine)) ;
  • Kim, Gon-Sup (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine)) ;
  • Park, Jung-Hee (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine)) ;
  • Hah, Dae-Sik (Gyeongnam Provincial Government Institute of Health and Environment) ;
  • Ryu, Jae-Doo (National Veterinary Research and Quarantine Service Pusan Regional Office) ;
  • Son, Sung-Gi (Gyeongnam Livestock Promotion Institute) ;
  • Heo, Jung-Ho (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Jung, Myung-Ho (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Kim, Jong-Shu (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine))
  • 김충희 (경상대학교 수의과대학(동물의학연구소)) ;
  • 김곤섭 (경상대학교 수의과대학(동물의학연구소)) ;
  • 박정희 (경상대학교 수의과대학(동물의학연구소)) ;
  • 하대식 (경상남도 보건 환경 연구원) ;
  • 류재두 (국립수의과학 검역원 부산지원) ;
  • 손성기 (경상남도축산진흥연구소) ;
  • 허정호 (경상남도 축산진흥연구소 남부지소) ;
  • 정명호 (경상남도 축산진흥연구소 남부지소) ;
  • 김종수 (경상대학교 수의과대학(동물의학연구소))
  • Accepted : 2002.04.19
  • Published : 2002.06.29

Abstract

Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk $C_{18}$($40{\mu}m$, 18% load, endcapped, 2g). A column made from the resultant $C_{18}$/animal tissue matrix was first washed with hexane($8m{\ell}$), following which the benzimidazoles were eluted with acetonitrile($8m{\ell}$). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear($0.991{\pm}0.007$ to $0.996{\pm}0.005$) with average relative percentage recoveries from $62.1{\pm}3.8(%)$ to $92.3{\pm}7.5(%)$ for the concentration range($0.2{\sim}6.4{\mu}g/g$), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2% and 83.5% at condition II, respectively. Condition II showed higher recoveries rates than condition I. These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable for the determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin.

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