Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Comparison of Antigenic Spots between Escherichia coli O157:H7 Strains by 2-Dimensional Gel Electrophoresis

이차원전기영동을 이용한 Escherichia coli O157:H7 균주간 항원 Spot의 비교

  • Ahn, Yeong-Chang (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Shin, Gee-Wook (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Shin, Yong-Seung (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Lee, Eung-Goo (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Lee, Hyoung-Jun (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Park, Mi-Rim (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Kim, Young-Rim (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Jung, Tae-Sung (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Kim, Gon-Sup (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University) ;
  • Kim, Yong-Hwan (Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University)
  • 안영창 (경상대학교 수의과대학 동물의학연구소) ;
  • 신기욱 (경상대학교 수의과대학 동물의학연구소) ;
  • 신용승 (경상대학교 수의과대학 동물의학연구소) ;
  • 이응구 (경상대학교 수의과대학 동물의학연구소) ;
  • 이형준 (경상대학교 수의과대학 동물의학연구소) ;
  • 박미림 (경상대학교 수의과대학 동물의학연구소) ;
  • 김영림 (경상대학교 수의과대학 동물의학연구소) ;
  • 정태성 (경상대학교 수의과대학 동물의학연구소) ;
  • 김곤섭 (경상대학교 수의과대학 동물의학연구소) ;
  • 김용환 (경상대학교 수의과대학 동물의학연구소)
  • Accepted : 2002.05.28
  • Published : 2002.06.29

Abstract

Proteomics is an emerging powerful tool in studying protein expression and function. At present study, proteomics was employed to evaluate the antigenicity among Escherichia coli O157:H7 strains using 2-dimensional gel electrophoresis (2-DE) and immunoblotting, SDS-PAGE and immunblotting analysis revealed no big differences among E coli O157:H7 strains. 2-DE analysis, however, revealed common antigens as well as specific antigens. The immunoblotting analysis revealed 20 common antigenic spots among E coli O157:H7 strains. In addition, there were 3 and 13 spots as common antigens between ATCC 43894 and KSC 109, and between ATCC 43894 and ACH 5, respectively. Antigenic spots specific for individual strain were also identified as 15, 8 and 22 for ATCC 43894, ACH 5 and KSC 109, respectively. The common antigens would be useful by employing either vaccine development or diagnosis marker, or both, whereas the specific antigens of individual strains would be applicable for epidemiological study. This study suggest that proteome analysis, representative as 2-DE, is valuable tool in exploring the E. coli antigenicity.

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Acknowledgement

Supported by : 한국과학재단

References

  1. McNeish AS, Turner P, Fleming J, et al. Mucosal adherence of human enteropathogenic Escherichia coli Lancet, 2(7942):946-948, 1975
  2. Truszczynski M, Pilaszek J. Pathogenic properties of Escherichia coli enterctoxin after parenteral administration to pigs, rabbits and mice. Res Vet Sci, 11(2):117-22, 1970
  3. Albert B. Manual of clinical microbiology, 5th ed. 367-370, 1991
  4. Garwim JL, Klages AL, Cronan JEJr. Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli. J Biol Chem, 255(24):11949-11956, 1980
  5. Cleary TG, Mathewson JJ, Faris E, et al. Shiga-like cytotoxin ptoduction by enteropathogenic Escherichia coli serogroups. Infect Immun, 47(1):335-337, 1985
  6. Rocchi G, Capozzi M. Enterohemorrhagic Escherichia coli 0157:H7 infection Recenti Prog Med, 90(11):613-618, 1999
  7. Pebody Rg, Furtado C, Rojas A, et al. An international outbreak of Vero cytotoxin-producing Escherichia coli 0157 infection amongst tourists; a challenge for the European infectious disease surveillance network Epidemiol Infect, 123(2):217-223, 1999
  8. Michino H, Araki K, Minami S, et al. Massive outbreak of Escherichia coli 0157:H7 infection in schoolchildren in Sakai City, Japan, associated with consumption of white radish sprouts. Am J Epidemiol, 150(8):787-796, 1999
  9. Konowalchuk J, Speirs JI, Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun, 18(3): 775-779, 1977
  10. Karmali MA, Petric M, Lim C, et al. The association between idiopathic hemolydc uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis, 151(5):775-782, 1985
  11. Pai CH, Ahmed N, Lior HV, et al. Epidemiology of sporadic diarrhea due to verocytctoxin-ptoducing Escherichia coli : a two-year prospective study. J Infect Dis, 157(5): 1054-1057, 1988
  12. Hockin JC and Lior H. Hemorrhagic colitis and hemolytic uremic syndrome caused by to Escherichia coli 0157:H7 in Canada. Can Dis Wkly ReP, 13, 203-204, 1987
  13. Read SC, Gyles CL, Clarke RC, et al. Prevalence of verocytotoxigenic Escherichia coli in ground beef, pork, and chicken in southwestern Ontario. Epidemiol Infect, 105(1): 11-20, 1990
  14. Suthienkul 0, Brown JE, Seriwatana J, et al. Shiga-like toxin producing Escherichia coli in retail meats and cattle in Thailand. Appl Environ Microbiol. 56(4):1135-1139, 1990
  15. Blattner FR. Plunkett G, Bloch CA, et al. The complete genome sequence of Escherichia coli K-12. Science, 277(5331):1453-1474, 1997
  16. Hayashi T, Makino K, Ohnishi M, et al. Complete genome sequence of enterohemorrhagic Escherichia coli 0157:H7 and genomic comparison with a laboratory strain K-12, DNA Res, 8(1):11-22, 2001
  17. Williams KL. Genomes and pooteomes: towards a multidimensional view of biology. Electrophoresis, 20(4-5):678-688, 1999
  18. O'Connor CD, Adams P, Alefoundo- P, et al. The analysis of microbial proteoms: Strategies and data exploitation. Electrophoresis, 21(6):1178-1186, 2000
  19. O' Farrell PH. High resolution two-dimensional electro phoresis of proteins. J Biol Chem, 250(10):4007-4021, 1975
  20. Blum H, Beier H, Gross HJ. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis, 8:93-99, 1987
  21. 정석찬. 국내 발견 신세대 식중독균의 문제점과 예방대책 Escherichia coli O157:H7의 특성 및 최근 연구동향. 한국식품안전성 학회 심포지엄, 1998
  22. Gevaert K, Vandekerckhove J. Protein identification methods in proteomcs. Electraphoresis, 21(6): 1145-1154, 2000
  23. Tonella L, Hoogland C, Binz P, et al. New perspectives in the Escherichia coli proteome investigation. Proteomics, 1(3):409-423, 2001
  24. Yokoyama, K, Iinuma Y, Kawano Y, et al. Resolution of Escherichia coli O157:H7 That Contaminated Radish Sprouts in Two Outbreaks by Two-dimsnsional Gel Electrophoresis, Current Microbiology, 43(5):311-315, 2001
  25. Cordwell SJ, Nouwens AS, Walsh BJ. Comparative proteomics of bacterial pathogens, Proteomics, 1(4):461-472. 2001