Evaluation on the radioprotective effect of Korean favorite teas

한국인 기호 차류의 방사선 장해 경감효과 평가

  • Kim, Se-Ra (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Hae-June (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Heon (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jin-Hee (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Hu-Kyung (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Tae-Hwan (Korea Cancer Center Hospital) ;
  • Jo, Sung-Kee (Korea Atomic Energy Research Institute) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2002.12.09
  • Published : 2002.12.30


We performed this study to determine the effect of Korean favorite teas (green tea, ginseng tea, coffee and barley tea) on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of ${\gamma}$-radiation. Jejunal crypts were protected by pretreatment of green tea (P.O.: 1.25% water extract, for 7 days before irradiation., I.P.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01) or ginseng (I.P.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.05). Green tea (p<0.05) or ginseng (p<0.05) administration before irradiation (I.P. at 12 and 36 hours before irradiation) resulted in an increase of t formation of endogenous spleen colony. The frequency of radiation-induced apoptosis was also reduced by pretreatment of green tea (P.O.: p<0.005, I.P.: p<0.05), pretreatment of ginseng (P.O.: p<0.005, I.P.: p<0.005) or posttreatment of ginseng (I.P.: 50 mg/kg of body weight, at 30 minutes after irradiation, p<0.05). Treatment with coffee or barley tea showed no significant modifying effects on the radiation-induced damages. These results indicated that green tea and ginseng might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the promotion nature of green tea, ginseng and its components.


Supported by : 과학기술부


  1. Eisenberg DM, Kessler RC, Foster C, et al. Unconventional medicine in the United States. Prevalence, costs, and patterns of use. N Engl J Med, 328:246-252, 1993
  2. Craig WJ. Health-promoting properties of common herbs. Am J Clin Nutr, 70:491S-499S, 1999
  3. IAEA safety series No. 47. Manual on Early Medical Treatment of Possible Radiation Injury. IAEA, Vienna, 74, 1978
  4. NCP repeat No. 65. Management of Persons Accidentally Contaminated with Radionuclides. 77, 1980
  5. Patt H, Tyree M and Straube RL. Cystein protects against x-irradiation. Science, 110:213-214, 1949
  6. Milas L, Hunter N, Reid BO, et al. Protective effects of S-2-(3-aminopropylamino) ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice. Cancer Res, 42:1888-1987, 1982
  7. Milas L, Murray D, Brock WA. et al. Radioprotectors in tumor radiotherapy: Factors and settings determining therapeutic ratio. Pharmacol. Ther, 39:179-189, 1988
  8. Neta R, Douches S and Oppenheim JJ. Interleukin 1 is a radioprotector. J Immunol, 136:2483-2485, 1986
  9. Neta R. Role of cytokines in radioprotection. Pharmacol Ther, 39:261-266, 1988
  10. MacVittie TJ, Monroy RL, Patchen ML, et al. Therapeutic use of recombinant human G-CSF (rhG-CSF) in a canine model of sublethal and lethal whole body irradiation. Int J Radiat Biol, 57:723-736, 1990
  11. Sweeney TR. A survey of compounds from the antiradiation drug development program of the U.S. army medical research & development command. Walter Reed Army Institute of Reseaich. Washington, DC, 1979
  12. Kligerman MM, Shaw MI, Slavid M. et al. Phase I clinical studies with WR2721. Cancer Clin Trials, 3:217-221, 1980
  13. 손경희, 이민준, 민성희, 등. 여성의 커피와 다류의 섭취에 영향을 주는 요인에 관한 연구. 한국식생활문화학회지. 15:398-412, 2000
  14. Washburn LC, Carlton JE and Hayes RL. Distribution of WR-2721 in normal and malignant tissue of mice and rats bearing solid tumors: dependence on tumor type, drug dose and species. Radiat Res, 59:483-575, 1974
  15. Cairnie AB. Adverse effect of radioprotector WR2721. Radiat Res, 94:221-226, 1983
  16. Yuan Y, Hou S, Lian T, etn al. Studies of Rehmannia glutinosa Libosch. f. hueichingensis as a blood tonic. Chung Kuo Chung Yao Tsa Chih, 17:366-368, 1992
  17. Miyanomae T and Frindel E. Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation. Exp Hematol, 16:801-806, 1988
  18. Wang Y and Zhu B. The effect of angelica polysaccharide on proliferation and differentiation of hematopoietic progenitor cell. Chung Hua I Hsueh Tsa Chih, 76:363-366, 1996
  19. Zneg XL, Li XA and Zhang BY. Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih, 12:607-608, 1992
  20. Wang HB, Zheng QY, Ju DW, et al. Effects of Phytolacca acinosa polysaccharides II on lymphocyte proliferation and colony stimulating factor production from mice splenocytes in vitro. Yao Hsueh Hsueh Pao, 28:490-493, 1993
  21. Hsu HY, Hau DM and Lin CC. Effects of kuei-pi-tang on cellular immunocompetence of gamma-irradiated mice. Am J Chin Med, 21:151-158, 1993
  22. Lu G, Yang M, Shen Y, et al. The absorption of Fe, Zn, Cu in siwu, sijunzi, and Liuwei dihuang decoction by small intestine in rats. Chung Kuo Chung Yao Tsa Chih, 16:297-298, 1991
  23. Mitscher LA, Jung M, Shankel D, et al. Chemoprotection: a review of the potential therapeutic antioxident properties of green tea (Camellia sinensis) and certain its constituents. Med Res Rev, 17:327-365, 1997
  24. Sato T and Miyata G. The nutraceutical benefit, Part I: green tea. Nutrition, 16:315-317, 2000
  25. Dufresne CJ and Farnworth ER. A review of latest research findings on the health promotion properties of tea. J Nutr Biochem, 12:404-421, 2001
  26. Cotelle N, Bernier JL, Henichart JP, et al. Scavenger and antioxidant properties of ten synthetic flavones. Free Radic Biol Med, 13:211-219, 1992
  27. Guo Q, Zhao B, Li M, et al. Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes. Biochim Biophys Acta, 1304:210-222, 1996
  28. Dreosti IE. Bioactive ingredients: antioxidants and polyphenols in tea. Nutr Rev, 54:S51-58, 1996
  29. Zloch Z. The role of dietary plant polyphenols in health maintenance. Cas Lek Cesk, 135:84-88, 1996
  30. Weisburger JH. Tea and health: the underlying mechanism. Proc Soc Exp Biol Med, 220:271-275, 1999
  31. 남기열. 최신 고려 인삼(성분 및 효능 편). 한국인삼연초연구원, 1996
  32. Yonezawa M. Restoration of radiation injury by intraperitoneal injection of ginseng extract in mice. J Radiat Res Tokyo, 17:111-113, 1976
  33. Takeda A, Yonezawa M and Katoh N. Restoration of radiation injury by ginseng. I. Responses of X-irradiated mice to ginseng extract. J Radiat Res (Tokyo), 22:323-335, 1981
  34. Yonezawa M, Katoh N and Takeda A. Restoration of radiation injury by ginseng. II. Some properties of the radioprotective substances. J Radiat Res (Tokyo), 22:336-343, 1981
  35. Yonezawa M, Katoh N, Takeda A. Restoration of radiation injury by ginseng. IV. Stimulation of recoveries in CFUs and megalaryocyte counts related to the prevention of occult blood appearance in X-irradiated mice. J Radiat Res (Tokyo) 26:436-442. 1985
  36. Takeda A, Katoh N, Yonezawa M. Restoration of radiation injury by ginseng. III. Radioprotective effect of thermostable fraction of ginseng extract on mice, rats and guinea pigs. J Radiat Res (Tokyo), 23:150-167, 1982
  37. Zhang JS, Sigdestad CP, Gemmell MA, et al. Modification of radiation response in mice by fractionated extracts of Panax ginseng. Radiat Res, 112:156-163, 1987
  38. Ben-Hur E and Fulder S. Effect of Panax ginseng saponins and Eleutherococcus senticosus on survival of cultured mammalian cells after ionizing radiation. Am J Chin Med, 9:48-56, 1981
  39. Kim SH. Cho CK, Yoo SY, et al. In vivo radioprotective activity of Panax ginseng and diethyldithiocarbamate. IN VIVO, 7:467-470, 1993
  40. Kim SH, Jeong KS, Ryu SY, et al. Panax Ginseng prevents apoptosis in hair follicles and accelerates recovery of hair inedullary cells in irradiated mice. IN VIVO, 12:219-222, 1998
  41. 조철구, 김태환, 류성렬, 등. 방사선 조사에 의한 DNA double strand breaks의 생성 및 회복에 미치는 인삼 알카로이드 분획의 효과. 대한치료방사선과학회지, 13:113-120, 1995
  42. George KC, Hebbar SA, Kale SP and Kesavan PC. Caffeine protects mice against whole-body lethal dose of gamma-irradiation. J Radiol Prot, 19:171-176, 1999
  43. Devasagayam TP and Kesavan PC. Radioprotective and antioxidant action of caffeine: mechanistic considerations. Indian J Exp Biol, 34:291-297, 1996
  44. Jee SH, He J, Appel LJ, et al. Coffee consumption and serum lipids: a meta-analysis of randomized contiolled clinical trials. Am J Epidemiol, 153:353-362, 2001
  45. Tavani A and La Vecchia C. Coffee and cancer: a review of epidemiological studies, 1990-1999. Eur J Cancer Prev, 9:241-256, 2000
  46. Whitfield FB. Volatiles from interactions of Maillard reactions and lipids. Crit Rev Food Sci Nutr, 31:1-58, 1992
  47. Krasner SW, McGuire MJ, Jacangelo JC, et al. The occurrence of disinfection by-products in US drinking water. J AWWA, 81:41-53, 1989
  48. Kim F and Lee S. Changes in the concentrations of the tap water chlorination by-products by heating during cooking and human ingestion exposure. Kor J Environ Toxicol, 14:35-43, 1999