Detection of Salmonella spp. by TaqMan real-time PCR and comparison of nucleotide sequences of ompC gene among Salmonella

TaqMan 실시간 중합 효소 연쇄반응에 의한 살모넬라속의 검출 및 ompC 항원단백 유전자의 비교

  • Lee, Young-Sung (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Choi, Kyoung-Seong (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Myeong-Chul (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Han, Jae-Cheol (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Chae, Joon-Seok (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University)
  • 이영성 (전북대학교 수의과대학 생체안전성연구소) ;
  • 최경성 (전북대학교 수의과대학 생체안전성연구소) ;
  • 김명철 (전북대학교 수의과대학 생체안전성연구소) ;
  • 한재철 (전북대학교 수의과대학 생체안전성연구소) ;
  • 채준석 (전북대학교 수의과대학 생체안전성연구소)
  • Accepted : 2002.12.04
  • Published : 2002.12.30

Abstract

Antigenic ompC genes of S. gallinarum, S. pullorum and S. dublin were characterized among Salmonella spp. isolated from chickens and other animals to identify genetic variation. Salmonella ompC gene fragment (1,027 bp) was amplified by PCR and the amplicons were cloned for comparison of nucleotide sequences. The identity of the sequences between S. gallinarum and S. pullorum, S. gallinarum and S. dublin, S. pullorum and S. dublin was 99.8%, 97.6% and 97.8%, respectively. Also, we found that ompC has some diversity between S. gallinarum and S. pullorum, and other Salmonella spp. which may be useful to type the organisms. Similar to diagnosis in other organisms, the TaqMan PCR method can be applied to rapid and accurate diagnosis of salmonellosis in chickens and other animals. We designed PCR primers and TaqMan probe for flagellin gene (fliC) for detection of Salmonella spp. by TaqMan PCR. The TaqMan PCR method was 10,000 times more sensitive than conventional PCR.

Acknowledgement

Supported by : 한국학술진흥재단

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