The Relationship Between the NF-${\kappa}B$ Activity and Anti-inflammatory Action of Surfactant in the Acute Lung Injury of Rats

백서의 급성폐손상에서 surfactant의 항염증작용과 호중구의 NK-${\kappa}B$ 활성과의 관계

  • An, Chang-Hyeok (Department of Internal Medicine, ChungAng University College of Medicine) ;
  • Cha, Young-Joo (Department of Laboratory Medicine, ChungAng University College of Medicine) ;
  • Lee, Kyoung-Hee (Department of Internal Medicine Seoul National University College of Meicine) ;
  • Yoo, Chul-Gyu (Department of Internal Medicine Seoul National University College of Meicine) ;
  • Lee, Byoung-Jun (Department of Internal Medicine, ChungAng University College of Medicine) ;
  • Jeong, Do-Young (Department of Internal Medicine, ChungAng University College of Medicine) ;
  • Lee, Sang-Hoon (Department of Internal Medicine, ChungAng University College of Medicine) ;
  • Shin, Jong-Wook (Department of Internal Medicine, ChungAng University College of Medicine) ;
  • Kim, Jae-Yeol (Department of Internal Medicine, ChungAng University College of Medicine) ;
  • Park, In-Won (Department of Internal Medicine, ChungAng University College of Medicine) ;
  • Choi, Byoung-Whui (Department of Internal Medicine, ChungAng University College of Medicine)
  • 안창혁 (중앙대학교 의과대학 내과학교실) ;
  • 차영주 (중앙대학교 의과대학 진단검사의학과교실) ;
  • 이경희 (서울대학교 의과대학 내과학교실) ;
  • 유철규 (서울대학교 의과대학 내과학교실) ;
  • 이병준 (중앙대학교 의과대학 내과학교실) ;
  • 정도영 (중앙대학교 의과대학 내과학교실) ;
  • 이훈 (중앙대학교 의과대학 내과학교실) ;
  • 신종욱 (중앙대학교 의과대학 내과학교실) ;
  • 김재열 (중앙대학교 의과대학 내과학교실) ;
  • 박인원 (중앙대학교 의과대학 내과학교실) ;
  • 최병휘 (중앙대학교 의과대학 내과학교실)
  • Published : 2002.11.30

Abstract

Background : The therapeutic effects of surfactants on acute lung injury derive not only from their recruiting action on collapsed alveoli but also from their anti-inflammatory action in the alveolar sapce. This study evaluated the anti-inflammatory action of a surfactant in an acute lung injury model of rats by neutrophils were recollected from the BAL fluid and the NF-${\kappa}B$ activity of the neutrophilic nuclear protein was evaluated. Methods : Male Sprague-Dawley rats weighing approximately 300 gram were divided into 3 groups, which consisted of 6 rats respectively. In the control group, normal saline(3ml/kg) was instilled into the trachea twice with 30 minute interval. In two other groups, acute lung injury was induced by the intra-tracheal instillation of LPS(5mg/kg). Thirty minutes later, either a surfactant(ST group; 30mg/kg) or normal saline(NT group: 3ml/kg) was instilled via the trachea. Twenty-four hours after the LPS instillation, the BAL fluid was retrieved to measure the WBC count and cytokine(IL-$1{\beta}$ and IL-6) levels. The neutrophils were isolated from the BAL fluid and the nuclear protein was extracted to evaluate the NF-${\kappa}B$ activity using a eletrophoretic mobility shift assay(EMSA). Results : The WBC count of the BAL fluid of the ST group($3,221{\pm}1,914{\times}10^3/{\mu}l$) was higher than that of the control group($356{\pm}275{\times}10^3/{\mu}l$)(p<0.05) and lower than that of the NT group($5,561{\pm}1,757{\times}10^3/{\mu}l$)(p<0.05)). The BAL fluid level of IL-$1{\beta}$ from the NT group($2,064{\pm}1,082pg/ml$) was higher than those of the ST group($360{\pm}234pg/ml$)(p<0.05) and the control group(0pg/ml)p<0.05) and control group($49{\pm}62pg/ml$)(p<0.05). The NF-${\kappa}B$ activity of the neutrophilic nuclear protein in the ST group and NT group was similar. Conclusion : The surfactant, attenuates the alveolar inflammation in the acute lung injury of rats model. However, its anti-inflammatory action does no't appear to be mediated by the inhibition of NF-${\kappa}B$ activity.