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Poly(DL-Lactide-co-Glycolide) Nanoparticles Used PEG-PPG Diblock Copolymer by Surfactant: Preparation and Loading of Water Insoluble Drug

유화제로서 PEG-PPG 블록 공중합체를 이용한 Poly(DL-Lactide-co-Glycolide) 나노입자: 제조 및 지용성 약물의 로딩

  • 정택규 (한국화학연구원 화학소재연구부) ;
  • 김승수 (한국화학연구원 화학소재연구부) ;
  • 신병철 (한국화학연구원 화학소재연구부)
  • Published : 2003.10.20

Abstract

In this study, poly(DL-lactide-co-glycolide) nanoparticles loaded with water-insoluble vitamins such as vitamin A (Retinol) and vitamin E acetate were prepared by the emulsification diffusion method. Polymer solution was prepared by the two water-miscible organic solvent, such as ethanol and acetone. Because of its biocompatible property, polyethyleneglycol-polypropyleneglycol diblock copolymer was used as surfactant and stabilizer. The influence of some preparative variables on the nanoparticle formation and on the loading efficiency of active agents, such as the type and concentration of stabilizing agent, the stirring methods, the water/oil phase ratio and the polymer concentration were investigated in order to control and optimize the process. After preparation of nanoparticles loaded with active agent, particle size and distribution were evaluated by the light scattering particle analyzer. The loading efficiency of active agents was evaluated by the UV-visible spectroscopy. As the results, particle size were 50-200 nm and dispersibility was monodisperse. The optimum loading efficiency of active agents was observed 50-60%. It was found that the appropriate of selections of binary solvent mixtures and polymeric concentrations in both organic and aqueous phases could provide good yield and favorable physical properties of PLGA nanoparticles.

Keywords

PLGA; Nanoparticle; Emulsification; Vitamin

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