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Detection of p53 Mutation in Colorectal Cancer Using PCR-SSCP and DHPLC

대장암에서 PCR-SSCP와 DHPLC를 이용한 p53 돌연변이의 검출

  • Sang-Bum Park (Department of Chemistry, College of Natrural Science, Dankook University) ;
  • Sang-Man Han (Department of Chemistry, College of Natrural Science, Dankook University) ;
  • Youn-Hyoung Nam (Department of Chemistry, College of Natrural Science, Dankook University) ;
  • Won-Cheoul Jang (Department of Chemistry, College of Natrural Science, Dankook University)
  • 박상범 (단국대학교 자연과학대학 화학과) ;
  • 한상만 (단국대학교 자연과학대학 화학과) ;
  • 남윤형 (단국대학교 자연과학대학 화학과) ;
  • 장원철 (단국대학교 자연과학대학 화학과)
  • Published : 2003.10.20

Abstract

Structural alteration of p53 and overexpression of p53 protein are the most common genetic abnormalities in various kinds of human cancer. Mutations in the p53 tumor-suppressor gene are usually associated with an advanced development of colorectal cancer characterized by the transition from the adenoma to carcinoma stage. Mutations in exons 5-8 of the p53 gene were analyzed by the polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) and denaturing high performance liquid chromatography(DHPLC). SSCP analysis detected 7 mutations(C13109>T) in 50 colorectal cancer samples(14%) at exon 5, and DHPLC analysis detected 7 mutations (C13109>T) and 2 mutation(C13202>A, C13204>G) in 50 colorectal cancer samples(18%) at exon 5. All of 9 mutations were proved by sequencing analysis. We conclude that DHPLC is a highly sensitive and specific method for p53 gene mutations.

Keywords

p53; Colorectal Cancer; PCR-SSCP; DHPLC,;Mutation

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