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Development of Diagnostic Strip for Determining Paraquat in Urine

뇨중 파라쿼트(paraquat)측정용 진단 시험지의 개발

  • Park Sang-Bum Park (Department of Chemistry, College of Natural Science, Dankook University) ;
  • Won-Cheoul Jang (Department of Chemistry, College of Natural Science, Dankook University) ;
  • Jong-Wan Kim (Department of Laboratory Medicine, Dankook University Medical College)
  • 박상범 (단국대학교 자연과학대학 화학과) ;
  • 장원철 (단국대학교 자연과학대학 화학과) ;
  • 김종완 (단국대학교 의과대학 진단검사의학과)
  • Published : 2003.08.20

Abstract

Paraquat is an effective and widely used herbicide, but it is also very toxic to humans. It is well-known that urine paraquat concentration is one of the most important prognostic indicator for paraquat-poisoning. Quantitative analysis of paraquat, however, are not generally used in clinical laboratories. In this work, a new test strip to detect paraquat concentration using sodium dithionite in urine was developed. Using these second-derivative method, the test strip prepared in $0.5{\%}$ borate buffer (pH 8.0), 0.25 M $Na_2S_2O_4,\0.1~0.8{\%}$ PVP, and $1{\%}$ decanol showed not only better color reaction but also an excellent application possibility to be used in automatic analyzer.

Keywords

Paraquat;Diagnostic strip;Urine

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