Separation and Determination of Co(II) and Ni(II) Ion as their 4-(2-Pyridylazo) resorcinol Chelates by Reversed-Phase Capillary High-Performance Liquid Chromatography

역상 모세관-고성능 액체 크로마토그래피에 의한 코발트와 니켈 이온의 4-(2-피리딜아조)레조루신올 킬레이트로서의 분리 및 정량

  • Published : 2003.12.20


Separation and determinations of Co(II) and Ni(II) ions as their 4-(2-pyridylazo)resorcinol(PAR) chelates by reversed-phase capillary high-performance liquid chromatography(RP-CpHPLC) were performed. Among many capillary columns, Vydac C4 column was selected and acetonitrile solution was used as mobile phase. The effect of pH and MeCN concentration(%) on the retention factor, k and peak intensity was examined and discussed. As a results, it was found that 22.5% MeCN and pH 5.60 was adequate as mobile phase for the separation of the two metal ions and determination of Co(II) ion, but the mobile phase condition for Ni(II) ion determination was 22.5% MeCN of pH 7.20. Detection limit(D.L., S/N=3) of Co(II) and Ni(II) ions were $2.0{\times}10{-7}$ M(14.9 ppb) and $1.0{\times}10{-6}$ M(59.2 ppb), respectively.


Reversed-Phase Capillary-HPLC;Cobalt(II) ion;Nickel(II) ion;4-(2-Pyridylazo) Resorcinol (PAR)


  1. Chervet, J. P.; Ursem, M.; Salzman, J. P.; Vannoort, R.W. LC/GC Magazine, 1989, 7, 514.
  2. Chang, H. C. K.; Taylor, L. T. Anal. Chem., 1991, 63, 486.
  3. Chervet, J. P.; van Soest, R. E. J.; Salzman, J. P. LC/GC Intl., 1992, 5, 33.
  4. Ranalder, U. B.; Lansecker, B. B.; Huselton, C. J. Chromatogr., 1993, 617, 129.
  5. Yeung, B.; Vouros, P. J. Chromatogr., 1993, 645, 115.
  6. Kassel, D. B.; Shushan, B.; Sakuma, T.; Salzrnzn, J. P. Anal. Chem. 1994, 66, 236.
  7. Okutani, T.; Sakuragawa, A.; Murakami, M. Anal. Sci., 1991, 7(Suppl.), 109.
  8. Chung, Y.; Lee, K Microchem. J., 2001, 69, 143.
  9. Hashem, E.Y. Spectrochim. Acta(Part I), 2002, 58A(7), 1401.
  10. Abbas, M. N.; Homoda, A. M.; Mostafa, G. A. E. Anal. Chim. Acta, 2001, 436(2), 223.
  11. Ghasemi, J.; Niazi, A. Microchem. J., 2001, 68, 1.
  12. Daryl, A.R Anal. Chem., 1984, 56, 241.
  13. Vachirapatama, N.; Doble, P.; Haddad, P. R. J. Chromatogr. A, 2000, 885(1+2), 369.
  14. Wang, H.; Miao, Y. X.; Mou, W.; Zhang, H. S.; Cheng, J. K. Mikrochimica Acta, 1994, 117, 65.
  15. Lee, K. S.; Lee, D. W.; Chung, Y. Anal. Chem., 1973, 45, 396.
  16. Yotsuyanagi, T. Buseki Kagaku, 1985, 34, 583.