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Diagnosis of Bovine Theileriosis by Direct PCR and Electrophoresis from Whole Blood Without DNA Extraction

DNA 추출없이 전혈을 이용한 PCR-전기영동법에 의한소의 타일레리아병 진단

  • Seong Ho Kang (Department of Chemistry, Chonbuk National University) ;
  • Sangmin Jang (Department of Chemistry, Chonbuk National University) ;
  • Joon-Seok Chae (Bio-safety Research Institute, Chonbuk National University) ;
  • Yongseong Kim (Division of Chemistry and Chemical Engineering, Kyungnam University)
  • 강성호 (전북대학교 화학과) ;
  • 장상민 (전북대학교 화학과) ;
  • 채준석 (전북대학교 생채안전성연구소) ;
  • 김용성 (경남대학교 정밀화학공학부)
  • Published : 2003.04.20

Abstract

We have developed a direct polymerase chain reaction (PCR) and electrophoresis method for the diagnosis of bovine theileriosis from whole blood DNA analysis without DNA extraction. The technique empolyed a FoLT (formamide, low temp.) technique and was utilized in the diagnosis of bovine theileriosis. Formamide solubilize the blood cells, and the lowered incubation temperatures reduced protein coagulation. 100-200 nL of whole blood and PCR reagents were introduced directly into a PCR tube. After the amplification, the PCR product (816-bp DNA) was introduced into the electrophoresis system. The results of this analysis were consist with those obtained using purified DNA.

Keywords

T. buffeli(buffeli/orientalis/sergenti);Direct PCR;Whole Blood;Diagnosis

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