Isolation and Identification of Pathogenic Bacteria from Spinach

시금치로부터 병원성세균의 분리 및 동정

  • Kim, Hye-Jung (Division of Life Sciences, Kyungnam University) ;
  • Kim, Young-Hoon (Gyeongsangnam-do Provincial Government Institute of Health and Environment) ;
  • Lee, Dong-Sun (Division of Life Sciences, Kyungnam University) ;
  • Paik, Hyun-Dong (Division of Life Sciences, Kyungnam University)
  • Published : 2003.02.01


Raw and washed spinaches were tested to evaluate the incidences of Aeromonas hydrophila, Escherichia coli O157:H7, Plesiomonas shigelloides, Pseudomonas aeruginosa, Salmonella spp., Shigella spp., Yersinia enterocolitica, Bacillus cereus, Campylobacter jejuni, Clostridium perfringens, Listeria monocytogenes, and Staphylococcus aureus. Four pathogenic bacteria were isolated from spinach samples, and identified by morphological and biochemical methods, including API and ATB identification systems. Isolates from MacConkey, Cereus Selective, Clostridium Perfringens, and Baird-Parker agar media were in 99.9, 99.8, 99.9, and 97.8% agreements with A. hydrophila, B. cereus, C. perfringens, and S. aureus at the species level, respectively. SET-RPLA revealed, among the five strains of S. aureus isolates, two produced type A enterotoxin. All five strains of B. cereus isolates produced enterotoxin as revealed with CRET-RPLA.


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