Adipocyte Differentiation Inhibitor Isolated from the Barks of Phellodendron amurense

황백(Phellodendri Cortex)으로부터 분리한 지방세포 분화 저해물질

  • Kim, Kyung-Hee (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ahn, Soon-Cheol (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Myung-Sun (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kweon, Oh-Song (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Oh, Won-Keun (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Min-Soo (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Sohn, Cheon-Bae (Department of Food and Nutrition, Chungnam National University) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Published : 2003.06.01


For the development of the anti-obesity natural drug, the inhibitor of adipocyte differentiation was screened from Korean traditional medicinal plants. Phellodendri Cortex was selected as a candidate of adipocyte differentiation inhibitor. An inhibitory compound PC-4 was purified from the methanol (MeOH) extract of Phellodendri Cortex using silica gel and ODS RP-18 column chromatography and HPLC. PC-4 was obtained as yellow powder; UV ${\lambda}_{max}$ (MeOH): 230, 260, 340 and 430 nm. The chemical structure of PC-4 was determined as an isoquionoline alkaloid, berberine, on the basis of various NMR experiments including $^1H-\;and\;^{13}C-NMR$. The PC-4 inhibited the differentiation of preadipocyte NIH-3T3 L1 cells at a concentration of $1\;{\mu}g/mL$.


Phellodendri Cortex;inhibitor;adipocyte differentiation;Phellodendron amurense;berberine


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