Analysis of Genetically-Modified Soybean and Soybean Sprout by Enzyme-Linked Immunosorbent Assay (ELISA)

효소면역측정법을 이용한 유전자재조합 콩과 콩나물의 분석

  • Kwak, Bo-Yeon (Food Function Research Division, Korea Food Research Institute) ;
  • Ko, Seung-Hee (Food Function Research Division, Korea Food Research Institute) ;
  • Shin, Won-Sun (Food Function Research Division, Korea Food Research Institute) ;
  • Shon, Dong-Hwa (Food Function Research Division, Korea Food Research Institute)
  • 곽보연 (한국식품개발연구원 식품기능연구본부) ;
  • 고승희 (한국식품개발연구원 식품기능연구본부) ;
  • 신원선 (한국식품개발연구원 식품기능연구본부) ;
  • 손동화 (한국식품개발연구원 식품기능연구본부)
  • Published : 2003.08.01

Abstract

It was determined whether the sandwich ELISA using specific anti-CP4 EPSPS polyclonal and monoclonal antibodies, developed in the previous study, could be applied to detect GM soybean or not. The soybeans (47 imported and 20 domestic soybeans) were analyzed by a sandwich ELISA. The results of imported soybeans were divided into two groups which were high contents $(39.1{\pm}13.5\;{\mu}g/g,\;n=33)$ and low contents of CP4 EPSPS $(2.6{\pm}1.2\;{\mu}g/g,\;n=14)$. The ratio of GM in imported soybeans was about 70.2%. One the other hand, the contents of CP4 EPSPS in domestic soybeans was very low $(0.9{\pm}0.5\;{\mu}g/g,\;n=20)$ which determined to be non-GM soybeans. In case of soybean sprouts, the contents of CP4 EPSPS in soybean sprouts were different between GM and non-GM soybean sprout. The CP4 EPSPS in cotyledon of GM soybeans sprout was higher than that in root hair. The contents of CP4 EPSPS in soybeans sprout of domestic soybeans were very low. Thus, it was possible to determine that the soybeans sprout was made of GM or non-GM soybeans. Also, PCR experiment showed that the sandwich ELISA was accurate to distinguish the soybeans to be GM or non-GM. These results showed the sandwich ELISA could determine the soybeans were GM or non-GM, rapidly and simply.

Keywords

GM soybeans;sandwich ELISA;CP4 EPSPS

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