An Improved Method for Determination of Catechin and Its Derivatives in Extract and Oil of Grape Seeds

포도씨유 및 추출물의 카테킨류 측정방법 개선

  • Moon, Sung-Ok (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Lee, Jun-Young (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Kim, Eun-Jung (Division of Drug Standardization, Korea Food and Drug Administration) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu)
  • 문성옥 (대구가톨릭대학교 식품영양학과) ;
  • 이준영 (대구가톨릭대학교 식품영양학과) ;
  • 김은정 (식품의약품안전청 약품규격과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과)
  • Published : 2003.08.01

Abstract

An improved method in place of a conventional vanillin spectroscopic method (CVSM) was developed for determination of catechin and its derivatives in extract and oil of grape seeds. For the CVSM, grape seed extracts had relatively high catechin content in the range of $17{\sim}43%$ (g/100g of extract), while grape seed oils had relatively smaller catechin content in the range of $30{\sim}40mg%$ (mg/100g of oil). For the improved vanillin spectroscopic method (IVSM) using a polyamide cartridge, catechin content of grape seed extracts was in the range of $4.0{\sim}7.5%$, while that of grape seed oils was below 5 ppm. Meanwhile, the quantities of catechin and its derivatives were determined by HPLC in the grape seed extracts and oils. Four major catechins [(+)-catechin, procyanidin B2, (-)-epicatechin, and epicatechin gallate] were detected from grape seed extracts, and the ranges of concentrations were as follows: (+)-catechin, $1.35{\sim}2.60%$; procyanidin $B_2$, $0.77{\sim}1.36%$; (-)-epicatechin, $2.35{\sim}4.59%$; (-)-epicatechin gallate, $0.06{\sim}0.30%$. In contrast, four catechins were barely detectable in the grape seed oils. The reproducibility of four major catechins in grape seed extracts, given as coefficient of variation, was below 5%, and the recovery close to above 95%. The achieved detection level of four catechins was $1{\sim}5\;ppm$. Additionally, the contents of catechin compositions in grape seed extract were also determined by HPLC in relation to different cultivars and producing areas. Thus, HPLC method and IVSM using polyamide cartridge can be used as alternative to CVSM for determination of catechin and its derivatives in extract and oil of grape seeds.

Keywords

catechins determination;grape seed extract and oil;vanillin spectroscopic method;HPLC

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